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| 左归丸促进小鼠未成熟卵母细胞体外核成熟的实验研究 | |
| 引用本文： | 孙晓峰,罗令,孙启春,高玉强,贺又舜,陈北阳,吴霞.左归丸促进小鼠未成熟卵母细胞体外核成熟的实验研究[J].现代生物医学进展,2011,11(3):444-447. |
| 作者姓名： | 孙晓峰 罗令 孙启春 高玉强 贺又舜 陈北阳 吴霞 |
| 作者单位： | 1. 湖南中医药大学,湖南,长沙,410007 2. 湖南省长沙市第八医院,湖南,长沙,410000 3. 山东省枣庄市市立医院,山东,枣庄,277100 |
| 基金项目： | 湖南省教育厅资助项目，湖南省科技厅博士后专项 |
| 摘 要： | 目的:研究左归丸对小鼠未成熟卵母细胞体外核成熟的影响。方法:制备左归丸含药血清,将生发泡(germinal vesicle,GV)期卵母细胞分别在不同采血时间获取的左归丸含药血清培养液中进行体外培养,观察左归丸含药血清对生发泡破裂(germinal vesicle breakdown,GVBD)和第一极体(the first polar body,PB1)排出的时效关系。结果:药物血清组卵母细胞GVBD的发生率高于正常血清组和对照组,于培养后4h差异最显著(P<0.01);药物血清组卵母细胞PB1的发生率高于正常血清组和对照组,于培养后18h差异最显著(P<0.01)。结论:2～2.5h左归丸含药血清对未成熟卵母细胞体外核成熟具有明显促进作用。 |
| 关 键 词： | 左归丸 血清 卵母细胞 生发泡破裂 第一极体 |
Experimental Study of Zuogui Pills in Stimulation of Mouse Immature Oocytes Nuclear Maturation in Vitro |
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| SUN Xiao-feng,LUO Ling,SUN Qi-chun,GAO Yu-qiang,HE You-shun,CHEN Bei-yang,WU Xia.Experimental Study of Zuogui Pills in Stimulation of Mouse Immature Oocytes Nuclear Maturation in Vitro[J].Progress in Modern Biomedicine,2011,11(3):444-447. | |
| Authors: | SUN Xiao-feng LUO Ling SUN Qi-chun GAO Yu-qiang HE You-shun CHEN Bei-yang WU Xia |
| Institution: | SUN Xiao-feng1,LUO Ling2,SUN Qi-chun3,GAO Yu-qiang3,HE You-shun1,CHEN Bei-yang1,WU Xia1(1 Hunan University of TCM,Changsha Hunan 410007 China,2 Changsha Eighth Hospital,Changsha Hunan 410000 China,3 Zaozhuang Municipal Hospital of Shandong,Zaozhuang Shandong 277100 China) |
| Abstract: | Objective:To investigate the effect of Zuoguiwan in vitro on nuclear maturation of immature mouse oocytes.Methods:The serum containing Zuoguiwan were prepared.The immature oocytes were isolated from the ovaries of the mouse.The GV oocytes were cultured in the culture media supplemented with Zuoguiwan serum with different blood sampling time,respectively.The rates of GVBD and PB1 extrusion were detected.Results:There was higer GVBD in several Zuoguiwan serum groups than that in the controls and normal serum ... |
| Keywords: | Zuoguiwan Serum Oocyte Germinal vesicle breakdown Polar body |
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