| Paxillin在大鼠胰腺发育不同时期表达的研究 |
| |
| 引用本文： | 郭静,石慧,曹丽华,刘莉洁,张一鸣.Paxillin在大鼠胰腺发育不同时期表达的研究[J].现代生物医学进展,2008,8(1):4-6,15. |
| |
| 作者姓名： | 郭静 石慧 曹丽华 刘莉洁 张一鸣 |
| |
| 作者单位： | 南京医科大学生化与分子生物学系,南京,210029 |
| |
| 基金项目： | 江苏省高校自然科学基金资助(07KJD310139) |
| |
| 摘 要： | 目的:研究桩蛋白(Paxillin,Pxn)在胰腺发育不同阶段的表达和细胞定位.方法:运用RT-PCR技术检测Pxn在大鼠胰腺发育不同阶段的mRNA表达水平;运用免疫组织化学检测不同时期桩蛋白在胰腺的定位.结果:RT-PCR结果显示Pxn的mRNA表达量胚胎期高于新生和成年期;免疫组织化学结果显示在不同发育时期桩蛋白不仅在外分泌胰腺有表达,而且在胰岛也有表达.结论:具有调节细胞聚集、粘附迁移功能的桩蛋白可能参与出生后胰岛重塑.
|
| 关 键 词： | Paxillin 胰岛重塑 细胞迁移粘附 RT-PCR 免疫组织化学 Paxillin 大鼠 胰腺发育 不同发育时期 表达量 研究 Pancreas Development Expression 功能 迁移 粘附 细胞聚集 调节 胰岛 外分泌 免疫组织化学检测 成年期 新生 胚胎期 |
| 文章编号： | 1673-6273（2008）01-0004-03 |
| 收稿时间： | 2007-09-16 |
| 修稿时间： | 2007-10-28 |
Research on Expression of Paxillin in the Development of Rat Pancreas |
| |
| GUO Jing,SHI Hui,CAO Li-hua,LIU Li-jie,ZHANG Yi-ming.Research on Expression of Paxillin in the Development of Rat Pancreas[J].Progress in Modern Biomedicine,2008,8(1):4-6,15. |
| |
| Authors: | GUO Jing SHI Hui CAO Li-hua LIU Li-jie ZHANG Yi-ming |
| |
| Abstract: | Objective:To investigate the expression and the cell location of paxillin during pancreas development.Methods: RT-PCR and immunohistochemistry were used to check paxillin expressions on mRNA level and the location in pancreas respectively in different stage of pancreas development.Results:The expression of paxillin was found much higher in embryonic stage than newborn and adult stage.Paxillin localized both in islets and exocrine pancreas,which was detected by immunohistochemistry.Conclusion:With functions of regulating cell aggregation,migration and adhesion paxillin might play roles in islet remodeling after birth. |
| |
| Keywords: | Paxillin Islet remodeling Cell adhesion and migration RT- PCR Immunohistochemistry |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
