| 大鼠胰腺发育阶段Mesothelin的表达 |
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| 作者单位： | 南京医科大学生物化学与分子生物学系 |
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| 摘 要： | 目的:研究Mesothenlin在大鼠胰腺发育阶段的表达和细胞定位。方法:运用RT-PCR和Western Blot技术分别检测Mesothenlin在大鼠胰腺发育阶段的mRNA和蛋白表达水平;运用免疫荧光检测不同时期Mesothenlin在胰腺的组织细胞学定位。结果:RT-PCR结果显示E18.5 Mesothelin mRNA的表达水平显著增高,至P14达到高峰,成年较低。Western Blot结果显示其蛋白表达趋势与mRNA完全相同。免疫荧光结果显示在不同发育时期Mesothenlin与胰岛β细胞和间充质细胞共表达。结论:Mesothenlin在大鼠胚胎胰岛形成及生后结构重塑中出现显著性高表达,并表达于胰岛β细胞和间充质细胞。
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| 关 键 词： | Mesothelin RT-PCR Western Blot 免疫荧光 胰岛重塑 |
Expression of Mesothenlin in Rat Pancreas during Development |
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| Authors: | ZHANG Guo-ying HOU Liang-qin LIN Yang-bo GONG Hua WANG Yin-he ZHONG Xin-cun XU Rong XIA Biao TONG Hui WANG Ning DE Wei |
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| Abstract: | Objective:To research the expression and the cell location of Mesothenlin during rat pancreas development.Methods: RT-PCR,Western Blot and double fluorescence immunohistochemistry were used to check Mesothenlin expressions on mRNA and pro- tein levels and location in the pancreas islets respectively in different stages of rat pancreas development.Results:The expression of Mesothenlin was significantly high in E18.5,reached the peak in P14,and then decreased until adult rat pancreas.Mesothelin protein ex- pression tendency was consistent with the Mesothelin mRNA expression.Immunolocalization indicated that Mesothelin not only co-lo- calized with insulin in islet beta-cells but also with vimentin in mesenchyme of developing rat pancreas.Conclusion:Mesothenlin is ex- pressed significantly highly during the process of islet formation of rat embryo and structural remodeling after birth,expressing in islet beta-cells and mesenchymal cells in rat pancreatic islets. |
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| Keywords: | Mesothenlin RT-PCR Western Blot Immunofluorescence Isletremodeling |
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