罗格列酮联合胰岛素对糖尿病大鼠氧化应激及血脂水平的影响

摘要 目的：探讨罗格列酮（RSG）联合胰岛素（INS）对糖尿病大鼠的氧化应激和血脂水平的影响。方法：30只健康雄性Wistar大鼠，随机分为3组：健康对照组、DM模型组和RSG+INS组，每组10只。采用链脲佐菌素（STZ）造模法进行糖尿病大鼠模型诱导。此后以RSG （3 mg/kg/day）灌胃，INS （2.25 U/kg/day）皮下注射，均连续给药8周，对RSG+INS组大鼠进行干预治疗。每2周定期记录各组大鼠的体重和血糖值1次；药物干预治疗8周后，取各组大鼠血清，对血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）、糖化血红蛋白（HbA1c）和丙二醛（MDA）的含量以及超氧化物歧化酶（SOD）和谷胱甘肽（GSH）的活性进行检测；应用苏木素碱性复红苦昧酸（HBFP）染色分析心肌形态变化。结果：RSG联合INS组治疗糖尿病大鼠，导致血糖发生时间依赖性下降（均P<0.05）；药物干预处理8周后，RSG+INS组大鼠的血清TC、TG、LDL和HbA1c水平均显著低于DM模型组，而HDL显著高于DM模型组（均P<0.05）；与DM模型组相比，RSG+INS组大鼠的血清MDA含量显著下降，而SOD和GSH活性则显著升高（均P<0.05）；与DM模型组相比，RSG+INS组心肌缺血/氧程度减轻。结论：RSG联合INS可有效对抗糖尿病大鼠体内的氧化应激损伤，改善糖尿病大鼠的脂代谢异常情况，并对心肌组织有一定的保护作用。

Effects of Rosiglitazone Combined with Insulin on Oxidative Stress and Lipid Profile Level in Diabetic Rats

ABSTRACT Objective: To explore the effects of rosiglitazone (RSG) combined with insulin (INS) on oxidative stress and lipid profile level in diabetic rats. Methods: 30 healthy male Wistar rats were randomly divided into three groups: healthy control group, DM model group and RSG+INS group, with 10 rats in each group. Diabetic rats were induced by streptozotocin (STZ) model. Then, the rats in RSG+INS group were treated with RSG (3 mg/kg/day) via stomach perfusion and INS (2.25U/kg/day) via hypodermic injection, and administered continuously for 8 weeks. The body weight and blood sugar values of each group were recorded 1 time every 2 weeks. After 8 weeks of drug intervention, the levels of serum total cholesterol(TC), triglyceride(TG), low density lipoprotein (LDL), high density lipoprotein(HDL), glycosylated hemoglobin(HbA1c) and the malonaldehyde(MDA) were detected, and the activities of serum superoxide dismutase(SOD) and glutathione (GSH) were detected. The morphological changes of myocardium were analyzed by hematoxylin alkaline Fuchsin Acid (HBFP) staining. Results: RSG combined with INS treatment in diabetic rats resulted in a time-dependent decrease in blood glucos(P all <0.05). After 8 weeks of drug intervention, the levels of serum TC, TG, LDL, HDL and HbA1c of rats in RSG+INS group were significantly lower than those in DM model group (P all <0.05). Compared with the DM model group, the levels of serum MDA of the rats in RSG+INS group were decreased significantly, while the activities of SOD and GSH were increased significantly (P all <0.05). Compared with the DM model group, the myocardial ischemia/oxygenation in the RSG+INS group was lessened. Conclusion: RSG combined with INS can effectively combat oxidative stress in diabetic rats and improve lipid metabolism in diabetic rats, and it has a protective effect on myocardial tissue.