寿胎丸提高子宫树突状细胞的表达改善超促排卵大鼠子宫内膜容受性

摘要 目的：探究寿胎丸提高子宫树突状细胞的表达改善超促排卵(controlled ovary hyperstimulation，COH）大鼠子宫内膜容受性。方法：将大鼠随机分为对照组、模型组、寿胎丸（低、中、高剂量）组，用促性激素释放激素激动剂（gonadotropin releasing hormone agonist，GnRH-a）、尿促性腺激素（human menopausal gonadotropin，HMG）和人绒毛膜促性腺激素（human chorionic gonado-tropin，HCG）构建COH大鼠模型，寿胎丸低、中、高剂量组采用2.5、5和10 g/ kg/d寿胎丸灌胃。流式检测子宫内膜树突状细胞（Uterine dendritic call，uDC）表面特异性标记蛋白OX-62。Western blot检测CD34和血管内皮生长因子（vascular endothelial growth factor，VEGF）蛋白表达。结果：与对照组比较，模型组OX-62阳性表达率降低，CD34和VEGF表达明显降低（P<0.05）。与模型组比较，寿胎丸低、中、高剂量组OX-62阳性表达率上升，CD34和VEGF表达明显升高，呈剂量依赖性（P<0.05）。结论：寿胎丸提高子宫树突状细胞的表达改善超促排卵大鼠子宫内膜容受性。

Shou Tai Wan Improved Endometrial Receptivity in Rats with Controlled Ovarian Hyperstimulation by Increasing Expression of uDC

ABSTRACT Objective: To explore the effect of Shou Tai Wan in improvement of endometrial receptivity in rats with controlled ovarian hyperstimulation by increasing the expression of uDC. Methods: Rats were randomly divided into control, model, and low, medium and high dose of Shou Tai Wan groups. The intervention groups were treated with gonadotropin-releasing hormone agonist (GnRH-a), urogonadotropin (HMG) and human chorionic gonadotropin (HCG) to establish COH rat model. Low, medium and high dose of Shou Tai Wan groups were treated with Shou Tai Wan 2.5, 5, 10 g/kg/d, respectively. Flow cytometry was used to detect the specific marker protein OX-62 on the surface of uterine dendritic cells(uDC).Western blot was used to detect the proteins expression of CD34 and vascular endothelial growth factor (VEGF). Results: Compared with the control group, in the model group, the positive expression rate of OX-62 was decreased, while the expression of CD34 and VEGF were significantly decreased(P<0.05). Compared with the model group, the positive expression rate of OX-62 and the expression of CD34 and VEGF were significantly increased in the low, medium and high dose group of Shou Tai Wan(P<0.05). Conclusion: Shou Tai Wan improved endometrial receptivity in rats with controlled ovarian hyperstimulation by increasing the expression of uDC.