慢病毒介导MicroRNA-376b-3p对垂体腺瘤细胞增殖和凋亡的影响及机制探究

摘要 目的：探究慢病毒介导MicroRNA-376b-3p对垂体腺瘤增殖和凋亡的影响及其可能的机制。方法：以体外培养的人垂体腺瘤细胞系为研究对象，分别设立为MicroRNA-376b-3p mimics组(对照组)和慢病毒lenti-sh MicroRNA-376b-3p组(药物组)，采用MTT法检测MicroRNA-376b-3p对垂体腺瘤细胞增殖的影响，AnnexinV-FITC/PI双染法检测MicroRNA-376b-3p对垂体腺瘤细胞凋亡率的影响，Western blot法检测MicroRNA-376b-3p对高迁移率蛋白A2(HMGA2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达的影响。结果：(1) 慢病毒介导MicroRNA-376b-3p能够显著抑制垂体腺瘤细胞的增殖(P<0.05)；(2)在MicroRNA-376b-3p干预后12 h、24 h以及48 h，垂体腺细胞的凋亡率均明显升高(P<0.05)；(3)经MicroRNA-376b-3p转染后，Bax蛋白表达升高，Bcl-2、Caspase-3、Survivin以及HMGA2蛋白表达降低(P<0.05)。结论：慢病毒介导MicroRNA-376b-3p能够明显抑制垂体腺瘤细胞增殖，诱导其凋亡，分析其作用机制可能与下调HMGA2和Survivin表达及上调Bax蛋白表达有关。

Effect of Lentivirus-mediated MicroRNA-376b-3p on the Proliferation and Apoptosis of Pituitary Adenoma Cells and the Mechanism

ABSTRACT Objective: To investigate the effect of lentivirus-mediated microrna-376b-3p on the proliferation and apoptosis of pituitary adenomas and its possible mechanism. Methods: Taking human pituitary adenoma cell lines cultured in vitro as the research object, the microRNA-376b-3p mimics group (control group) and lenti-sh MicroRNA-376b-3p group (drug group) were established respectively, and the MTT method was used to detect MicroRNA-376b-3p on the proliferation of pituitary adenoma cells, the AnnexinV-FITC/PI double staining method was used to detect the effect of MicroRNA-376b-3p on the apoptosis rate of pituitary adenoma cells, and the Western blot method was used to detect the effect of MicroRNA-376b-3p on HMGA2, Bax, Caspase-3 protein expression. Results: (1) Lentivirus-mediated Microrna-376b-3p could significantly inhibit the proliferation of pituitary adenoma cells (P<0.05). (2) At 12, 24 and 48 h after microrna-376b-3p intervention, the apoptosis rate of pituitary gland cells in the control group was significantly lower than that in the drug group (P<0.05). (3) After transfection with microrna-376b-3p transformation, the expression of Bax protein increased, and the expression of Bcl-2, Caspase-3, Survivin and HMGA2 protein decreased (P<0.05). Conclusion: Lentivirus-mediated Microrna-376b-3p could significantly inhibit the proliferation of pituitary adenoma cells and induce apoptosis. The mechanism of microrna-376b-3p may be related to its targeted down-regulation of HMGA2 expression, up-regulation of Bax protein expression and regulation of survivin protein expression.