食管癌组织miR-21、miR-182表达与临床病理特征及预后的关系

目的:探讨食管癌组织微小RNA-21(miR-21)、微小RNA-182(miR-182)表达与临床病理特征及预后的关系。方法:选择2010年10月至2014年2月期间在我院治疗的120例食管癌患者为研究对象,采集患者的食管癌组织及癌旁组织,采用荧光定量PCR检测组织中miR-21、miR-182表达量,采用Kaplan-Meier法分析患者生存情况。结果:与癌旁组织相比,食管癌组织中miR-21、miR-182表达量明显升高(P<0.05)。食管癌组织中miR-21、miR-182表达均与TNM分期和淋巴结转移相关(P<0.05)。miR-21低表达患者的5年生存率明显高于高表达患者(P<0.05),miR-182低表达患者的5年生存率明显高于高表达患者(P<0.05)。结论:miR-21、miR-182表达量在食管癌中上调,与食管癌TNM分期和淋巴结转移相关。miR-21高表达以及miR-182高表达患者5年生存率下降,检测miR-21、miR-182表达量在食管癌患者预后预测中具有一定临床意义。

The Relationship between the Expression of miR-21 and miR-182 in Esophageal Carcinoma Tissues and the Clinicopathological Characteristics and Prognosis

ABSTRACT Objective: To investigate the relationship between the expression of miRNA-21 (miR-21) and miRNA-182 (miR-182) in esophageal cancer tissues and the clinicopathological features and prognosis. Methods: A total of 120 patients with esophageal cancer who were treated in our hospital from October 2010 to February 2014 were selected as research objects. Esophageal cancer tissues and adjacent tissues of the patients were collected. Expressions of miR-21 and miR-182 in tissues were detected by fluorescence quantitative PCR. Kaplan-meier method was used to analyze patient survival. Results: Expression levels of miR-21 and miR-182 in esophageal cancer tissues were significantly higher than that in paracancer tissues (P<0.05).The expressions of miR-21 and miR-182 in esophageal cancer tissues were correlated with TNM staging and lymph node metastasis (P<0.05). The 5-year survival rate of patients with low miR-21 expression was significantly higher than that of patients with high miR-21 expression (P<0.05). The 5-year survival rate of patients with low miR-182 expression was significantly higher than that of patients with high miR-182 expression (P<0.05). Conclusion: The expression of miR-21 and miR-182 are up-regulated in esophageal cancer, which are related to TNM stage and lymph node metastasis of esophageal cancer. The 5-year survival rate of patients with high miR-21 expression and high miR-182 expression decreased, the detection of the expression levels of miR-21 and miR-182 has certain clinical significance in predicting the prognosis of patients with esophageal cancer.