柴胡渗湿汤对哮喘大鼠血清中IL-5 及IL-13 影响的实验研究

目的:观察柴胡渗湿汤对哮喘大鼠血清中IL-5及IL-13含量的影响,探讨柴胡渗湿汤治疗哮喘的作用机制。方法:将84只雄性Wi star大鼠按随机数字表法分为正常对照组、模型对照组、地塞米松组、定喘汤组、柴胡渗湿汤低剂量组、柴胡渗湿汤中剂量组、柴胡渗湿汤高剂量组,每组12只,采用卵蛋白制作大鼠哮喘模型,予相应药物干预。用酶联免疫吸附试验(ELISA)法检测各组大鼠血清中IL-5及IL-13水平。实验数据采用SPSS11.5统计软件进行分析。结果:实验后各组死亡率比较均无差异,P>0.05;模型对照组与正常对照组血清中IL-5及IL-13含量水平有显著差异,表明大鼠哮喘模型存在着血清IL-5及IL-13含量异常增高的病理状态;与模型对照组相比较,各治疗组血清中IL-5及IL-13的含量明显降低,其中尤以地塞米松组、柴胡渗湿汤中剂量组和柴胡渗湿汤高剂量组的作用更明显。结论:柴胡渗湿汤治疗哮喘的作用机制与降低IL-5及IL-13的水平有关;上述作用具有一定的量效关系,但并不因给药剂量的增加而增加。

Study on the Effects of Radix Bupleuri Dampness-Percolating Decoction on Serum IL-5 & IL-13 in Rat Asthma Model

Objective: To investigate the effects of radix bupleuri dampness-percolating decoction on the IL-5 and IL-13 in the rat asthma model serum and its function mechanism of asthma treatment.Methods: Eighty-four healthy male Wistar rats were divided by random number method into normal control group,model control group,dexamethasone group,dingchuan decoction group,low dosage radix bupleuri dampness-percolating decoction group,medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group,with 12 in each group.Then the model of rat asthma was made with ovalbumin and intervened by relevant medicine.The IL-5 and IL-13 in rat serum from each group were tested by enzyme-linked immunosorbent assay(ELISA);experimental data were analyzed by SPSS11.5 statistical software.Results: Mortality ratio of each group after experiment had no difference,P>0.05.There existed significant difference between the model control group and the normal control group in terms of serum IL-5 and IL-13 content,which indicated a pathological state that serum IL-5 and IL-13 in rat asthma model abnormally increased.Compared with model control group,IL-5 and IL-13 in treatment groups’ serum obviously decreased,especially to the dexamethasone group,medium dosage radix bupleuri dampness-percolating decoction group and high dosage radix bupleuri dampness-percolating decoction group.Conclusion: The function mechanism of radix bupleuri dampness-percolating decoction for asthma treatment is related to reduction of the IL-5 and IL-13 level.A certain dose-response relationship existed in the said effect,but the effect does not become stronger with higher dosage.