扶正化瘀胶囊对鼠血吸虫肝纤维化的作用及机制探讨

目的:研究扶正化瘀胶囊对实验性血吸虫病肝纤维化的影响,探讨其相关作用机制。方法:70只昆明小鼠分为正常对照组(A)、模型组(B)、吡喹酮组(C)、INF-γ组(D)、扶正化瘀胶囊低剂量组(E)、中剂量组(F)、高剂量组(G),采用日本血吸虫尾蚴30条/只攻击感染小鼠建立血吸虫性肝纤维化模型。第6周用吡喹酮驱虫治疗后予扶正化瘀胶囊干预,第14周末观察血清透明质酸、层粘连蛋白、III型前胶原、IV型胶原水平,HE染色观察肝脏病理改变及虫卵结节变化,免疫组化检测PDGF-BB和I、III型胶原的表达。结果:扶正化瘀胶囊各剂量组肝脏病理损伤减轻,虫卵肉芽肿面积和数量明显减小;血清HA、LN、PC-III、C-IV水平显著下降(P<0.05);肝组织I、III型胶原和PDGF-BB表达明显低于模型组(P<0.05)。结论:扶正化瘀胶囊具有抗血吸虫性肝纤维化作用,降低肝组织PDGF-BB的表达可能是其抗纤维化作用机制之一。

Therapeutical Effects and Mechanisms of Fuzhenghuayu Capsule in Murine Schistosomiasis Liver Fibrosis

Objective: To investigate the therapeutic efficacy and the mechanism of Fuzhenghuayu Capsule on murine schistosomiasis liver fibrosis.Methods: Seventy Kunming mice were randomly divided into norma-l control group(A),model control group(B),Praziquantel group(C),positive control by INF-γ group(D),Fuzhenghuayu Capsule low dose group(E),Fuzhenghuayu Capsule middle dose group(F) and Fuzhenghuayu Capsule high dose group(G).The models of hepatic fibrosis induced by S.japonicurn were established by exposure of mice percutaneously through the tail to cercariae of S.japonicum.On 6 weeks after the infection were orally introduced with praziquantel.Then treatment with Fuzhenghuayu Capsule.Fourteen weeks after being infected,assayed the serum levels of Hyaluronic acid(HA),Laminin(LN),Procolagen Type III(PCIII) and Collagen Type IV(C-IV).HE staining and Immunohistochemical techniques were used to detected the histopathological changes and the expressions of PDGF-BB and type I,III collagen.Results :The histopathological changes and the areas of egg granuloma in Fuzhenghuayu Capsule groups were relieved significantly,The level of serum HA,LN,PC-III,C-IV were significantly decreased(P<0.05),the expressions of PDGF-BB,type I and III collagen were significantly lower than those in the model group B(P<0.05).Conclusion : The Fuzhenghuayu Capsule have obviously inhibitory effect on the hepatic fibrosis induced by Schistosoma japonicum infection.The mechanisms may be reduced the expression of PDGF-BB.