内质网应激预处理对人正常肝细胞缺氧再复氧损伤的保护作用

目的:研究内质网应激预处理对人肝细胞缺氧复氧损伤的保护作用。方法:将培养的人肝细胞分为4组:正常对照(C)组、细胞缺氧复氧损伤(H/R)组、内质网应激(ER)组、内质网应激预处理(ERP+H/R)组。收集各组细胞,以流式细胞仪检测细胞凋亡,Western-bloting及RT-PCR检测内质网应激特异蛋白GRP78表达水平,并通过透射电镜观察各组细胞超微结构改变。结果:ERP+H/R组细胞凋亡率明显低于H/R组(P<0.05),ER及ERP+H/R组GRP78蛋白表达明显高于H/R组(P<0.05)。结论:内质网应激预处理对肝细胞缺氧复氧损伤具有明显的保护作用,内质网应激特异性蛋白GRP78可能在肝细胞缺氧复氧损伤中作为一种关键性的保护蛋白出现。

Protective Effect of Endoplasmic Reticulum Stress Preconditioning on the HL-7702 Cell Injuried by Hypoxia-Reoxygenation

Objective: To study the protective effects on the endoplasmic reticulum stress preconditioning on human hepatic cell hypoxia-reoxygenation injury.Methods: Cultured human liver cells into four groups,namely normal control(C) group,hypoxia-reoxygenation(H / R) group,endoplasmic reticulum stress(ER) group,endoplasmic reticulum stress preconditioning(ERP + H / R) group.The cells were collected to detect apoptosis by flow cytometry,Western-bloting and RT-PCR detection of endoplasmic reticulum stress protein GRP78-specific expression,and ultrastructural changes of cells were observed by transmission electron microscopy.Results: ERP + H / R cell apoptosis was significantly lower than H / R group(P <0.05),ER and ERP + H / R group GRP78 protein expression was significantly higher than the H / R group(P <0.05).Conclusion: Endoplasmic reticulum stress preconditioning on hypoxia-reoxygenation injury of liver cells has a significant protective effect.Endoplasmic reticulum stress-specific protein GRP78 is a key protective protein in liver cells injured by hypoxia-reoxygenation.