RhoB/ROCK 蛋白分子在重度子痫前期的表达

目的:探讨RhoB/ROCK蛋白分子在重度子痫前期(severe preeclampsia,sPE)患者胎盘组织中表达水平以及意义。方法:取sPE产妇和正常产妇胎盘组织各20例,通过Western blot和免疫组化检测胎盘组织中Rho亚家族蛋白(RhoB、RhoC)及其Rho激酶即ROCK(ROCKI、ROCKII)蛋白的表达水平,应用Spearman等级相关分析法检验RhoB、RhoC与ROCKI、ROCKII蛋白表达水平的相关性。结果:RhoB、ROCKI、ROCKII蛋白在sPE表达水平增加(P<0.05);sPE患者胎盘组织中RhoB与ROCKI、ROCKII蛋白表达水平均呈正相关(r=0.793,r=0.901,P<0.05)。结论:RhoB及其与下游分子ROCKI、ROCKII构成的信号通路,在sPE的发病中可能发挥着重要的作用。

The Expression of RhoB/ROCK Molecules in Severe Preeclampsia

Objective: To investigate the expression of RhoB and ROCK in severe preeclampsia and explore its clinical implication.Methods: Western blotting and immunohistochemical staining methods were used to detect the expression of Rho proteins(RhoB,RhoC)and kinase ROCK(ROCKI,ROCKII)in the placenta tissue including 20 severe preeclampsia pregnancy patients and 20 healthy pregnant women as control group.The correlation of the expression of RhoB,RhoC and ROCKI,ROCKII were analyzed Spearman rank correlation analysis.Results: RhoB,ROCKⅠand ROCK II were more abundant in the placenta tissue of the severe preeclampsia than in the normal placent(P<0.05).In severe preeclampsia group,the expression of RhoB and ROCKI,ROCK II had positive relationship(r= 0.793,r=0.901,P<0.05).Conclusion: Signaling pathway constituted RhoB and its downstream molecules ROCKI,ROCK II,may play essential role in severe preeclampsia.