首页 | 本学科首页   官方微博 | 高级检索  
   检索      

NALP1 基因与骨肉瘤细胞的相关研究
引用本文:刘浩郭猛章浩王洋程平纪方.NALP1 基因与骨肉瘤细胞的相关研究[J].现代生物医学进展,2012,12(6):1040-1043.
作者姓名:刘浩郭猛章浩王洋程平纪方
作者单位:第二军医大学长海医院
摘    要:目的:探讨NALP1基因在骨肉瘤细胞株MG-63、U-2OS中的表达,以及高表达NALP1基因对于骨肉瘤细胞体外凋亡的影响。方法:使用RT-PCR、Western-blot法检测骨肉瘤细胞株MG-63、U-2OS中的mRNA及蛋白表达水平并与人成骨细胞株hFOB1.19比较。将NALP1基因转染质粒PcDNA3.1,将重组质粒转染骨肉瘤细胞,分成高表达基因组、空质粒组及对照组,加入抗肿瘤药物顺铂及甲氨蝶呤促使肿瘤细胞凋亡,使用流式细胞仪测定各组肿瘤细胞凋亡率。结果:通过统计分析,骨肉瘤细胞株MG-63、U-2OS中的mRNA及蛋白表达水平均低于人成骨细胞株hFOB1.19(P<0.05),NALP1高表达组的肿瘤细胞凋亡率明显高于空白质粒组及对照组。结论:上调骨肉瘤细胞株MG-63、U-2OS中的NALP1的表达量可以促进肿瘤细胞凋亡。

关 键 词:人成骨肉瘤细胞株MG-63、U-2OS  NALP1基因  细胞凋亡

Research on NALP1 Gene and Osteosarcoma Cell
LIU Hao,GUO Meng,ZHANG Hao,WANG Yang,CHENG Ping,JI Fang.Research on NALP1 Gene and Osteosarcoma Cell[J].Progress in Modern Biomedicine,2012,12(6):1040-1043.
Authors:LIU Hao  GUO Meng  ZHANG Hao  WANG Yang  CHENG Ping  JI Fang
Institution:(ChangHai hospital of Second Military Medical University,200433,Shanghai,China)
Abstract:Objective: To investigate the expression of NALP1 gene in osteosarcoma cell line MG-63,U-2OS and effect in vitro apoptosis about high expression of NALP1 gene for osteosarcoma cells.Methods: We use the methods of RT-PCR,Western-blot to assay the expression of mRNA and protein in osteosarcoma cell line MG-63,U-2OS and compare with human osteoblast cell line hFOB1.19.We transfected the NALP1 gene into plasmid PcDNA3.1,and the recombinant plasmid was.We divided the transfected osteosarcoma cells into three groups,highly expressed genes group,blank plasmid group and control group.Then we add the anticancer drugs cisplatin and methotrexate to promote tumor cell apoptosis.At last we use flow cytometry in each group to determine the apoptosis of tumor cell.Results: Through statistical analysis,we get the result that mRNA and protein expression in osteosarcoma cell line MG-63,U-2OS were lower than the human osteoblast cell line hFOB1.19(P <0.05),and apoptosis of highly expressed NALP1 gene group was significantly higher than the blank plasmid group and the control group.Conclusions: Increasing the expression of NALP1 gene in osteosarcoma cell line MG-63 and U-2OS can promote apoptosis of tumor cell.
Keywords:Osteosarcoma cell line MG-63  U-2OS  NALP1 gene  Apoptosis
本文献已被 CNKI 等数据库收录!
点击此处可从《现代生物医学进展》浏览原始摘要信息
点击此处可从《现代生物医学进展》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号