脐带华通胶间充质干细胞向Flk1 阳性细胞分化的研究*

目的:诱导脐带华通胶间充质干细胞向Flk1阳性细胞分化。方法:胶原酶法分离培养脐带华通胶间充质干细胞,第3代细胞以含2-巯基乙醇的分化培养基培养,应用RT-PCR和流式细胞仪从mRNA和蛋白水平检测Flk1阳性细胞分化水平。结果:脐带华通胶间充质干细胞Flk1mRNA及蛋白表达极低,分化培养基培养后表达上调,48h达高峰(P<0.05),之后表达降低。结论:2-巯基乙醇可诱导脐带华通胶间充质干细胞向Flk1阳性细胞分化,为从中分选Flk1阳性细胞进行进一步研究提供了依据。

Induce Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly Differentiate into Flk1-positive Cells

Objective: Induce mesenchymal stem cells from human umbilical cord Wharton’s jelly differentiate into Flk1-positive cells.Methods: Mesenchymal stem cells from human umbilical cord Wharton’s jelly(WJ-MSCs) were isolated by collagenase digestion and passage 3 cells were cultured by differentiation medium contained 2-mercaptoethanol,Flk1 mRNA were examined by RT-PCR,Flk1 protein were analyzed by flow cytometry.Results: Low expression of Flk1 mRNA and Flk1 protein were detected in WJ-MSCs.Flk1 expression increased after cultured by differentiation medium,and reached maximum after 48h culture,then declined.Conclusions: Mesenchymal stem cells from human umbilical cord Wharton’s jelly could be induced and differentiated into Flk1-positive cells by 2-mercaptoethanol,which can be used to select Flk1-positive cells in WJ-MSCs.