TLR-4 在慢性阻塞性肺病患者肺组织表达的研究

目的:观察COPD患者肺组织中TLR-4,IL-8,MUC5AC的表达,并探讨其在气道炎症、气道高分泌中的作用。方法:非COPD、COPD组男性肺癌病人各20例,取其肺叶切除后的外周肺组织,对肺组织标本行HE及AB-PAS染色,用免疫组织化学方法检测肺组织中TLR-4,IL-8,MUC5AC的表达并分析其相关性。结果:①COPD患者肺组织中TLR-4,IL-8,MUC5AC表达较对照组增高(P<0.05)。TLR-4主要在气道上皮细胞、肺巨噬细胞及血管内皮细胞表达,IL-8在气道壁、肺泡间隔、血管壁及肺组织内浸润的单核细胞、巨噬细胞、多形核白细胞均有表达,MUC5AC主要在气道上皮杯状细胞中表达。②TLR-4、IL-8表达与气道炎细胞评分成正相关(P<0.05)。TLR-4与IL-8、MUC5AC表达成正相关(P<0.05)。结论:COPD患者肺组织中TLR-4高表达可能参与了COPD的气道炎症及气道高分泌,这可能是通过增加IL-8与MUC5AC的表达来实现的。

The Expression of TLR-4 in Patients with Chronic Obstructive Pulmonary Disease

Objective: To test the expression of TLR-4 in patients with chronic obstructive disease,and to explore the role of TLR4 in airway innammation and mucus hypersecretion of COPD pathogenesis.Methods: Lung specimens from patients without COPD(nonCOPD group) and patients with COPD(COPD group).20 in each group were investigated.All lung specimens was observed by HE and AB-PAS staining.The expression of TLR-4,IL-8,MUC5AC were observed by immunohistochemical staining.Results: ①The expression of TLR-4,IL-8 and MUC5AC in COPD group were higher than those in control group(P<0.05).TLR-4 major express in airway epithelial cells,pulmonary macrophage and vascular endothelial cells.IL-8 express in airway walls,alveolar interval,hemal wall and mononuclear cells,macrophages,nucleation white blood cells which infiltrate in lung issue.MUC5AC mainly express in the goblet cells of airway epithelial.②TLR-4,IL-8 were positively correlated with airway inflammatory cells score(P<0.05).TLR-4 was positively correlated with IL-8and MUC5AC(P<0.05).Conclusion: The high expression of TLR-4 probably involved in airway innammation and mucus hypersecretion of COPD pathogenesis.This may be achieved by increasing the expression of IL-8 and MUC5AC.