| 5- 氮杂-2'－脱氧胞苷对卵巢癌细胞系CP70 顺铂耐药性的逆转作用 |
| |
| 引用本文： | 张琳琳辛晓燕,张建芳白露高静.5- 氮杂-2'－脱氧胞苷对卵巢癌细胞系CP70 顺铂耐药性的逆转作用[J].现代生物医学进展,2012,12(9):1644-1647. |
| |
| 作者姓名： | 张琳琳辛晓燕 张建芳白露高静 |
| |
| 作者单位： | 第四军医大学西京医院妇产科 陕西西安710032 |
| |
| 摘 要： | 目的:探究5-氮杂-2’-脱氧胞苷体外逆转卵巢癌铂类耐药细胞系CP70对顺铂的耐药性,并探讨与SOCS-2表达的关系。方法:免疫细胞化学和Western blot方法检测使用5-氮杂-2’-脱氧胞苷处理细胞前后细胞内SOCS-2表达水平。MTT法检测单独使用5-氮杂-2’-脱氧胞苷或顺铂及两药联合使用对CP70细胞的抑制作用。结果:两药联合处理CP70细胞后顺铂耐药逆转倍数为1.34、1.63、2.34,联合处理组的细胞中SOCS-2表达明显升高。结论:5-氮杂-2’-脱氧胞苷可以部分逆转CP70细胞对顺铂的耐药性,且此作用可能与增加细胞的SOCS-2表达有关。
|
| 关 键 词： | 5-氮杂-2’-脱氧胞苷 卵巢癌 顺铂 耐药性 |
5-Aza-CdR Resensitizes Cisplatin-resistant Human
Ovarian Carcinoma Cells CP70 |
| |
| ZHANG Lin-lin,XIN Xiao-yan,ZHANG Jian-fang,BAI Lu,GAO Jing.5-Aza-CdR Resensitizes Cisplatin-resistant Human
Ovarian Carcinoma Cells CP70[J].Progress in Modern Biomedicine,2012,12(9):1644-1647. |
| |
| Authors: | ZHANG Lin-lin XIN Xiao-yan ZHANG Jian-fang BAI Lu GAO Jing |
| |
| Institution: | (Department of Obstetrics and Gynecology,Xijing Hospital,Fourth Military Medical University,Shanxi,Xi’an,710032,China) |
| |
| Abstract: | Objective:To investigate the effect of 5-Aza-CdR on resensitizing cisplatin-resistant human ovarian carcinoma ceils CP70 and discuss the related mechanism.Methods: Immunohistochemical analysis and Western blot were used to detect the expression of SOCS-2 in different groups.MTT assay was used to assess the inhibitory effects of 5-Aza-CdR,cisplatin alone or a combination of both on CP70 cells.Results: The resensitizing fold of cisplatin combined with 5-Aza-CdR was 1.34,1.63,2.34.The expression of SOCS-2 had the most prominent decrease in CP70 cells in the combination group.Conclusion: 5-Aza-CdR can partlly resensitize cisplatin-resistant CP70 cells,which might be related to the down-regulation of SOCS-2. |
| |
| Keywords: | 5-Aza-CdR Ovarian carcinoma Cisplatin Drug resistance |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 |
| 点击此处可从《现代生物医学进展》下载免费的PDF全文 |
