联合应用NGF 和胰岛素对糖尿病大鼠烫伤创面表皮干细胞的影响

目的:通过局部联合应用神经生长因子和胰岛素对糖尿病大鼠深II度烫伤创面表皮干细胞标记物β1整合素和角蛋白19(K19)表达的影响,探讨神经生长因子和胰岛素联合应用于糖尿病烫伤创面治疗后对创面愈合的影响。方法:雄性wistar大鼠腹腔注射链脲佐菌素(STZ)建立糖尿病模型60只,1月后在大鼠背部造成深II度烫伤。将大鼠随机分为糖尿病对照组(B)、胰岛素治疗组(C)、神经生长因子治疗组(D)、神经生长因子联合胰岛素治疗组(E),每组15只。另取15只正常雄性wistar大鼠作为正常对照组(A)。观察伤后3、7、11、15、21 d各组创面愈合情况,检测创面β1整合素和角蛋白19(K19)的表达并计算创面愈合率。结果:E组创面愈合率自第7天起较A、B、C、D组增加,为(25.33±2.32)%,(P<0.05)];A、C、D组创面愈合率较B组增加分别为(22.51±1.78)%,(16.68±1.95)%,(18.29±1.70)%,(P<0.05)]。E组整合素β1和角蛋白19(K19)表达自伤后第7至21天各时相点显著增加,(P<0.05)。结论:糖尿病大鼠深II度烫伤创面局部联合应用神经生长因子和胰岛素可促进表皮干细胞的增殖与分化从而加速创面的愈合。

Effects of Nerve Growth Factor Combination with Insulin on Wound Healing with II Degree Burn and the Regeneration of Epidermis Cells in Diabetic Rats

Objective: To investigate the effects of local application of NGF combine with insulin on wound healing with II degree deep scald and the expression of skin stem cells markers β 1 integrin and keratin 19(K19) in diabetic rats.Methods: 60 male Wistar rats were used in the study by intraperitoneal injection of streptozotocin(STZ).Deep partial thickness scalding was engendered on the back of the rats after one month.Then these rats were randomly divided into B(diabetes control,n=15),C(insulin treatment,n=15),D(NGF treatment,n=15) and E(NGF and insulin treatment,n=15)groups,and group A(nomal control,n=15) was created by normal rat of partial thickness scalding.The wound area,wound healing rate,and express of skin stem cells markers β 1 integrin and keratin 19(K19) immunohistochemistry staining were investigated after 3,7,15,and 21 day post scalding days(PSDs).Results: Compared to those in groups A、B、C and D,the wound healing rate in group E(25.33±2.32)% increased significantly since the 7th PSD(P<0.05).The wound healing rate in group A C and D increased significantly compare with group B (22.51±1.78)%,(16.68±1.95)%,(18.29±1.70)%,(P<0.05)].The expression of β 1 integrin and keratin 19(K19) in group E increased significantly compare with other groups from 7th day till 21st day(P<0.05).Conclusion: Local application of NGF combine with Insulin could be beneficial to promote skin stem cell proliferation and differentiation in the burn wounds of the rats with diabetes and accelerate wound healing.