rhG-CSF 动员对供者CD4+ T 细胞免疫表型和功能影响

目的:研究重组人粒细胞集落刺激因子(rhG-CSF)动员对供者CD4+T细胞表面分子淋巴细胞功能相关抗原-1(LFA-1)、细胞间黏附分子-1(ICAM-1)、L-选择素(LAM-1)和人整合素-4(VLA-4)的表达及其介导的CD4+T细胞功能的影响,探讨外周血干细胞移植过程中CD4+T细胞免疫耐受机制。方法:使用三色荧光标记检测动员前及动员后第5天供者外周血LFA-1、ICAM-1、LAM-1和VLA-4的表达率,ELISA方法检测动员前后CD4+T细胞分泌IFN-γ和IL-4能力,免疫磁性分选法分离纯化CD4+T细胞,检测动员前后CD4+T细胞对基质细胞衍生因子-1α(SDF-1α)的迁移能力和对ICAM-1的黏附能力。结果:动员前后CD4+T细胞LFA-1(CD11a)和VLA-4(CD49d)表达率差异无统计学意义(P>0.01),动员前后CD4+T细胞LAM-1(CD62L)和ICAM-1(CD54)的表达率差异均有统计学意义,动员前显著高于动员后(P<0.01);动员前后CD4+T淋巴细胞向SDF-1α的迁移率差异无统计学意义(P>0.01);动员后CD4+T细胞对ICAM-1的黏附率降低(P<0.01);动员后IL-4和IFN-γ两个细胞因子在外周血血清的浓度均降低(P<0.01)。结论:rhG-CSF动员不影响CD4+T细胞LFA-1和VLA-4表达及CD4+T细胞迁移,但影响CD4+T细胞ICAM-1和LAM-1表达以及CD4+T细胞通过LFA-1对ICAM-1的黏附能力影响,并可能影响CD4+T细胞分泌细胞因子IL-4及IFN-γ的功能。

Impact on Immunophenotypes and Functions of CD4+ T Cell During Mobilization in Donors with rhG-CSF

Objective: To investigate the mechanism of CD4+ T cells immunotolerance during allogeneic peripheral blood stem cell transplantation by studying the impact of recombinant human granulocyte colony stimulating factor(rhG-CSF) on expression of lymphocyte function antigen-1(LFA-1),intercellular adhesion molecule(ICAM-1),L-Selectin(LAM-1) and very late antigen 4α(VLA-4) on CD4+ T cells,and functions of CD4+ T cells through these molecules during mobilization.Methods: The expression of LFA-1,ICAM-1,LAM-1 and VLA-4 of CD4+ T cells were determined by FACS before rhG-CSF mobilization and the fifth day of mobilization.The expression of IFN-γ and IL-4 by CD4+ T cells were examined with ELISA.At the same time,the migration and adhesion activity of CD4+ T cells to SDF-1αand ICAM-1 were detected.Results: The expression of LFA-1 on CD4+ T lymphocytes were both 100%.The expression of VLA-4 didn’t change markedly before and the fifth day of mobilization(P>0.01).The expression of LAM-1(CD62L) and ICAM-1(CD54) on CD4+ T lymphocytes decreased during mobilization(P<0.01).SDF-1α induced CD4+ T lymphocytes migration for 4 hours didn’t change markedly during mobilization(P>0.10).The adhesion activity of CD4+T lymphocytes to ICAM-1 decreased markedly during mobilization(P>0.10).Conclusion: The concentration of IL-4 and IFN-γ in peripheral blood serum decreased markedly during mobilization(P<0.01).In conclusions,rhG-CSF does not have effect on the expression of LFA-1 and VLA-4 on CD4+ T cell,and the migration activity of CD4+ T cell during mobilization,but can affect the expression of ICAM-1and LAM-1 on CD4+ T cell,and the adhesion activity of CD4+ T cell to ICAM-1 through LFA-1.