| 高效液相色谱法测定经舒胶囊中丹皮酚的含量 |
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| 引用本文： | 朱韵洁,杨晓,徐红,谢宜名,杨钊,孙勇.高效液相色谱法测定经舒胶囊中丹皮酚的含量[J].现代生物医学进展,2012,12(11):2163-2166. |
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| 作者姓名： | 朱韵洁 杨晓 徐红 谢宜名 杨钊 孙勇 |
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| 作者单位： | 1. 青岛大学医学院药学系 山东 青岛 266021
2. 青岛市药品检验所 山东 青岛 266071 |
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| 基金项目： | project(81072398)supported by the National Natural Science Foundation of China~~ |
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| 摘 要： | 目的:建立以高效液相色谱法测定经舒胶囊中丹皮酚含量的方法。方法:采用Platisil ODS色谱柱(4.6 mm×250 mm 5μm);以甲醇-水(45:55)为流动相;柱温:40℃;流速:1.0ml.min-1;检测波长:274nm。结果:在建立的色谱条件下,丹皮酚进样量在0.08487~0.50922μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为98.75%(RSD=1.74%)。结论:方法简便可靠,分离度好,可用于经舒胶囊的质量控制。
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| 关 键 词： | 高效液相色谱法 丹皮酚 经舒胶囊 |
Determination of Paeonol in Traditional Chinese Medicine 'Jing-Shu'
Capsule by HPLC |
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| ZHU Yun-jie,YANG Xiao,XU Hong,XIE Yi-ming,YANG Zhao,SUN Yong.Determination of Paeonol in Traditional Chinese Medicine 'Jing-Shu'
Capsule by HPLC[J].Progress in Modern Biomedicine,2012,12(11):2163-2166. |
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| Authors: | ZHU Yun-jie YANG Xiao XU Hong XIE Yi-ming YANG Zhao SUN Yong |
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| Institution: | 1(1 College of Pharmacy,Qingdao university,Qingdao 266021,China;2 Qingdao Institute for Drug Control,Qingdao 266071,China) |
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| Abstract: | Objective: To establish the HPLC method for the determination of paeonol in ’JingShu’ Capsule.Methods: The separation was performed on Platisil ODS(250 mm×4.6 mm,5 μm)column;The mobile phase was methanol-water(45:55) at a flow rate of 1.0ml.min-1;The detection wavelength was 274 nm,and the column temperature was 40℃.Results: The calibration curve showed good linearity in the range of 0.08487~0.50922 μg(r=0.9999).The average recovery was 98.75%,RSD was 1.74%.Conclusion: The method is convenient,rapid and accurate.It could be used for control the quality of ’JingShu’ Capsule. |
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| Keywords: | HPLC Paeonol ’JingShu’ Capsule |
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