地塞米松对哮喘豚鼠血清白细胞介素-4 变化及肺组织形态的影响

目的:观察地塞米松对哮喘豚鼠血清白细胞介素-4(IL-4)及肺组织形态的影响,探讨糖皮质激素治疗哮喘的机制。方法:30只健康雄性Hartley系豚鼠随机分为空白对照组,病理模型组,地塞米松组。用卵白蛋白(OVA)复制哮喘豚鼠模型。采用流式细胞术分析CD4+interleukin-4(IL-4)细胞占CD4+T细胞的比例,光镜检查肺组织形态变化。结果:病理模型组外周血中IL-4明显高于空白对照组(P<0.05),地塞米松组外周血中IL-4较病理模型组明显降低(P<0.05),且地塞米松组支气管上皮损伤,粘液腺增生等病理改变较病理模型组明显改善。结论:地塞米松治疗哮喘的作用与抑制Th2的活化,改善支气管上皮损伤,粘液腺增生等病理改变有关。

Effects of Dexamethasone on Serum IL-4 and the Pathological Changes of Lung Tissue of Guinea Pig with Asthma

Objective: To observe the influence of dexamethasone on serum IL-4 and the pathological changes of lung tissue of guinea pig with asthma.and to explore the mechanism of dexamethasone in the treatment of asthma.Methods: 30 healthy male Hartley guinea pigs were divided into three groups randomly and equally: control group,model group,dexamethasone treatment group.The model guinea pigs of asthma was induced by intraperitoneal injection of ovalbumin.The level of CD4+ IL-4 was tested by flow cytometer and analyzed statistically.Lung histological changes were examined by HE stains and under microscopy.Results: IL-4 increased obviously in model group compared with control group(P<0.05).The levels of IL-4 in serum of dexamethasone treatment groups were significantly lower than those of model group(P<0.05).The bronchial tube epider-mis damage and the mucous membrane gland proliferation were obviously improved in dexamethasone treatment group compared with model group.Conclusion: Dexamethasone could relieving asthma according to inhibiting the activation of Th2 and improveing the lung tectology changes.