miR-30b 的组织特异性研究

目的:研究miR-30b的组织特异性,检测其在心,肝,脑,肾,脾和骨骼肌中的表达情况。方法:选取C57BL/6J雄性小鼠6只,用real-time PCR方法检测小鼠心,肝,脑,肾,脾和骨骼肌中miR-30b的表达量。结果:miR-30b在小鼠肝脏中表达量最低,与肝相比,在心,脑,肾,脾和骨骼肌中的相对表达量分别为13.13±0.899,9.497±0.717,4.478±1.031,6.751±0.596,2.538±0.79。且与肝相比均具有统计学意义(P<0.05)。结论:miR-30b在各组织中的表达存在差异,在心脏中的表达量最高,提示miR-30b可能与心脏的发生发展密切相关,为深入探索miR-30b的功能奠定了基础。

A Research on the Tissue-Specific of miR-30b

Objective: To investgate the tissue-specific of miR-30b and to detect the expression of miR-30b in hearts,livers,brains,kidneys,spleens and skeleton muscles of mice.Methods: The Real-time Quantitative PCR method were used to detect the expression levels of miR-30b in hearts,livers,brains,kidneys,spleens and skeleton muscles of 6 male C57BL/6J mice.Results: The expression level of miR-30b in livers of mice was the lowest.Compared the expression level in livers,the relative expression levels of miR-30b in hearts,brains,kidneys,spleens and skeletal muscles were respectively 13.13±0.899,9.497±0.717,4.478±1.031,6.751±0.596,2.538±0.79.They all had significant differences compared with the expression level of livers(P<0.05).Conclusion: The expression levels of miR-30b were different in various tissues,which had the highest expression in hearts.This suggests that miRA-30b may be closely related to the growing and development of hearts.It lays a foundation for exploring the function of miR-30b in depth.