上海交通大学医学院附属新华医院崇明分院检验科

目的:探讨富血小板血浆(platelet-rich plasma,PRP)结合组织工程皮肤对裸鼠巨大创面修复的影响。方法:首先,利用密度梯度离心法制备富含生长因子的浓缩血小板的血浆,并测其所含生长因子的量;其次,在裸鼠的背侧部分构建大面积皮肤创面,分别用人工真皮,组织工程皮肤,碱性成纤维细胞生长因子组织工程皮肤,表皮生长因子组织工程皮肤和PRP结合组织工程皮肤修复裸鼠巨大创面;最后,手术后不同时间间隔收集组织标本,采用HE染色,PAS染色和免疫组化等方法评估创面愈合情况。结果:PRP结合组织工程皮肤组创面修复愈合情况最好。

Combining Platelet-Rich Plasma and Tissue Engineer Skin in the Treatment of Large Skin Wound on Nude Mice

Objective: To investigate the effects of tissue engineered skin in combination with PRP and other preparations on the repair of large skin wound on nude mice.Methods: Platelet-rich plasma(PRP) from venous blood wase prepared by density gradient centrifugation.Large skin wounds were created surgically on the dorsal part of nude mice.The wounds were then treated with either artificial skin,tissue engineered skin,tissue engineered skin combined with basic fibroblast growth factor,and tissue engineered skin combined with epidermal growth factor or tissue engineered skin combined with PRP.Tissue specimens were collected at different time intervals post surgery.HE,PAS stains and immunohistochemistry were performed to assess rate of wound healing.Results: Macroscopic observations,HE/PAS staining and immunohistochemistry revealed that the tissue engineering skin in combination with the PRP group showed the most satisfactory wound recovery out of the five groups.