血清脂联素与载脂蛋白A5 及2 型糖尿病的关系

目的:检测血清脂联素(APN)水平,分析血清APN浓度与血脂、血清载脂蛋白A5(apoA5)及2型糖尿病的关系。方法:收集2型糖尿病(T2DM)210例,健康体检者112例,采用ELISA法检测血浆脂联素水平,双抗体夹心ELISA法检测血清载脂蛋白A5(apoA5)水平,7600-020E全自动生化分析仪检测总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)等,放射免疫分析仪检测血胰岛素水平。结果:T2DM患者血清APN浓度明显低于健康对照组,LDL-C、TG及TC均高于对照组(P<0.05)。T2DM患者血清apoA5浓度(200.3±51.2)ng/ml,显著低于健康对照组(229.8±56.5)ng/ml,P<0.05。Pearson相关分析显示经年龄、性别校正后APN水平与LDL-C、TG呈负相关,与HDL-C呈正相关;T2DM组APN与apoA5呈正相关(P<0.05)。结论:T2DM患者血清APN水平显著降低,本研究证实低水平血清APN和apoA5不仅与血脂代谢密切相关,还可作为T2DM患者早期监测的指标,对其预后评价具有积极的意义。

The Relationship Among Plasma Adiponectin, Apolipoprotein A5 and Type 2 Diabetic Patients

Objective: To test plasma adiponectin of type 2 diabetic patients(T2DM) and evaluate whether plasma adiponectin(APN) was associated with T2DM.Methods: Plasma APN were measured using adiponectin ELISA kit in 223 cases T2DM patients and 112 cases healthy examinations,apolipoproteinA5(apoA5) concentration were tested by sandwich enzyme-linked immunosorbent assay.Plasma total cholesterol(TC),triglycerin(TG),low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) were measured by 7600-020E automatic biochemistry analyzer,The serum insulin were measured by using RIA.Results: Plasma APN in T2DM were significantly lower than those in the control subjects,and the level of LDL-C,TG,Lp(a) are higher than the comparison group(P<0.05) Serum apoA5 concentrations(200.3±52.6)ng/ml of T2DM were lower than healthy control(229.8±56.5)ng/ml,P<0.05.Pearson correlation analysis showed that adiponectin was significantly negatively correlated with LDL-C and TG,and positively correlated with HDL-C after adjustment for age and sex,APN was positively correlated with apoA5 in T2DM.Conclusion: Serum APN of T2DM patients were apparently decreased,this research lower APN and apoA5 not only correlated with lipid metolism,but also as a early index for monitoring of T2DM patients,have active significance for Prognostic evaluation.