测定胰腺癌组织微血管密度及淋巴管密度的临床意义

目的:研究胰腺癌组织中微血管密度(Microvessel density,MVD)和淋巴管密度(Lymphatic vessel density,LVD)的变化、与胰腺癌临床病理的联系。方法:采用免疫组织化学SABC法应用CD34及D2-40分别检测41例胰腺导管腺癌患者配对癌组织内、癌旁及正常胰腺组织中MVD及LVD的表达情况,分析其与肿瘤分化程度、分期和淋巴转移间的相关性;以及癌组织MVD与癌旁组织LVD表达之间的关系。结果:在41例胰腺癌组织配对癌组织及正常胰腺组织平均MVD值分别为46.585±16.935,11.100±4.036,两组之间差别有统计学意义(P=0.000<0.01)。配对癌组织内、癌旁及正常胰腺组织中平均LVD值分别为11.244±4.800,15.829±7.470和13.512±5.139;其中癌旁组织与正常胰腺组织LVD值差别无统计学意义(P=0.060>0.05),癌旁组织与胰腺癌组织的LVD值有显著性差异(P=0.000<0.01)。癌组织内MVD值与癌旁组织LVD值之间有相关性(P=0.025<0.05)。结论:胰腺导管腺癌组织中MVD及LVD与肿瘤分化程度、病理分期及淋巴转移存在关联。胰腺癌组织内MVD值与癌旁组织LVD之间存在相关性。

Clinical Significances of Measuredvalue of Microvessel Density and Lymphatic Vessel Density in Pancreatic Carcinoma Tissue

Objective: To investigate the changes of the microvessel density(MVD) and Lymphatic vessel density(LVD) and their relationship with the clinical pathology parameer and their interaction.Methods: The MVD and LVD were detected by using CD34 and monoclonal antibody D2-40 in 41 patients with pancreatic ductal adenocarcinoma was detected by using immunohistochemical method SABC.The expression of MVD and LVD in the cancer,paraneoplastic and normal pancreatic tissue were analyzed.The relationship with the clinical pathology parameter like as the pathological stage,tumor differentiation and lymph node metastasis,and the correlation between the MVD of cancer tissues and LVD of paraneoplastic were analyzed.Result: The mean MVD value in paired cancer and normal pancreatic tissue were 46.585±16.935,11.100±4.036 respectively.There was statistically significant difference between the two groups(P=0.000<0.01).The mean MVD value in paired cancer and normal pancreatic tissue were 46.585±16.935,11.100±4.036 respectively.There was statistically significant difference between the two groups(P=0.000<0.01).The average LVD of cancer,paraneoplastic and normal pancreatic tissue were 11.244±4.800,15.829±7.470 and 13.512±5.139 respectively in 41 cases,and there was significant differerce between the paraneoplastic and cancer tissue(P=0.000<0.01) and contrary between paraneoplastic and normal pancreatic tissue(P=0.060>0.05).There was relationship between the MVD values of tissues and the LVD values of paraneoplastic tissues(P=0.025<0.05).Conclusion: The MVD values and LVD values of pancreatic ductal adenocarcinoma were associated with the tumor differentiation,pathological stage and lymph node metastasis.The MVD values of pancreatic cancer tissues were correlated with the LVD values of paraneoplastic tissues.