常压氧与高压氧对成年大鼠脑缺血再灌注损伤后 微血管新生影响的比较

目的:研究常压氧与高压氧对成年大鼠脑缺血再灌注损伤后微血管新生影响的差异。方法:将成年SD雄性大鼠随机分为三组:假手术组(SS组)、常压氧治疗组(NBO组)、高压氧治疗组(HBO组),每组又随机分为3、7、10天三个亚组。采用线栓法对NBO组和HBO组大鼠进行大脑中动脉栓塞(MCAO),缺血1.5小时后拔出栓子再灌注,NBO组进行常压氧治疗,HBO组进行高压氧治疗。大鼠分别在3、7、10天麻醉处死,取脑组织切片,血管内皮生长因子(VEGF)、VEGF受体-1(FLT-1)和CD34免疫组化染色,光镜观察取图和统计分析。结果:NBO各组与SS各组相比,VEGF、FLT-1和CD34阳性细胞数目均明显增多(P<0.05);HBO各组与NBO各组比较,7天、10天组VEGF、FLT-1和CD34阳性细胞数目均显著增多(P<0.05)。结论:HBO治疗较NBO治疗对成年大鼠微血管的新生更有促进作用。

Comparison of Normobaric Oxygen and Hyperbaric Oxygen on Angiogenesis in the Brain of Adult Rats with Cerebral Ischemia-Reperfusion Injury

Objective: To investigate the difference between normobaric oxygen(NBO) and hyperbaric oxygen(HBO) on angiogenesis in the brain of adult rats with cerebral ischemia-reperfusion injury.Methods: Adult male Sprague-Dawley(SD) rats were randomly divided into three groups: the sham surgery(SS) group,the NBO group and the HBO group.Each group was randomly divided into three subgroups: the 3 days group,the 7 days group and the 10 days group.Middle cerebral artery occlusion(MCAO) was performed to the NBO and HBO groups to develop the animal model.1.5 hours later,reperfusion was made.Then,the NBO group received NBO treatment,and the HBO group received HBO treatment.All the rats were sacrificed respectively on day 3,7,10;Cerebral samples were collected and prepared to sections.The sections were treated with immunohistochemical staining with the following antibody: Vascular endothelial growth factor(VEGF),VEGF receptor-1(Flt-1),CD34.The changes in ischemia region were detected under the optical microscope,and the pictures were taken.Results: Compared with those in the SS group,numbers of VEGF and Flt-1 and CD34 positive cells increased markedly in the NBO group(P<0.05).The numbers of VEGF and Flt-1 and CD34 positive cells increased significantly on day 7 and day 10,following HBO treatment compared with those in the NBO group(P<0.05).Conclusions: HBO therapy is better to promote angiogenesis in the brain of adult rats with ischemia-reperfusion injury than NBO treatment.