西安市MRSA 耐消毒剂基因qacA/B 的检测

目的:了解西安市三甲综合医院耐甲氧西林金黄色葡萄球菌(MRSA)qacA/B基因的携带情况,以便掌握其对消毒剂的耐药情况。方法:收集临床分离的152株耐甲氧西林金黄色葡萄球菌,采用聚合酶链反应检测qacA/B基因,并将qacA/B基因扩增产物进行测序,测序结果进行比对。结果:152株耐甲氧西林金黄色葡萄球菌中有11株检出qacA/B阳性,qacA/B携带率7.2%;测序结果比对发现,4株qacA的基因编码序列同一位点均有一个碱基发生突变(C→T),苏氨酸突变为异亮氨酸。结论:该地区临床分离的耐甲氧西林金黄色葡萄球菌中qacA/B携带率相对较低,但也存在抵抗消毒剂的风险,临床应加强消毒剂使用的管理,阻止MRSA的传播,减少感染的发生。

A Investigation of Disinfectant-Resisitant Gene QacA/B in Meticillin-Resisitant Staphylococcus Aureus Isolated from Xi'an's Hospital

Objective:To investigate the disinfectant-resistant gene qacA/B in meticillin-resisitant Staphylococcus aureus isolated from Xi’an’s hospital,in order to know the aureus ’s sensitivity of disinfectant.Methods: A total of 152 MRSA strains were isolated.Polymerase chain reaction(PCR) was implied to analyze the prevalence of gene qacA/B in these stains.Last,taking the positive-qacA/B gene’s output into testing the DNA sequence and analyzing.Results: Of the 152 MRSA strains,11 were positive for qacA/B gene.The prevalence of gene qacA/B in these stains was 7.2%.One nucleotide take place missense mutation(C→T)in some site at the coding section of qacA/B gene sequence of 4 strains.Conclusions: There were not very high positive percentages of relevant resistant gene qacA/B in MRSA isolated from clinical,but we also take seriously on the phenomenon of disinfectant resistance and make good use of disinfectants to prevent MRSA transferring,even more to decrease the prevalence of infection.