Bacillus spp.的筛选鉴定及浸矿渣效果研究

目的:研究Bacillus spp.的生长特性及浸镉渣效果。方法:采用9K培养基划线分离纯化目标菌种,利用传统的测定方法测定其生长特征,采用16S rDNA对菌株进行分子生物学鉴定,最后对该菌株浸三种矿渣浸出效果进行了分析。结果:该微生物形态为扁球形,革兰氏染色呈阳性,能运动,单生鞭毛;最适生长温度30℃,最适pH值为3;代谢类型为兼性营养型;耐受NaCl浓度为4%;微生物氧化酶为阴性,接触酶为阳性;经鉴定该菌株属于Bacillus属。以5%的接种量,培养基pH为3条件下,镉浸出效果较好,沉渣中浸出率为90.4%,其余两种渣中浸出效果最佳。结论:Bacillus spp.可应用于镉渣中镉的浸出。

Study on Screening and Identification of Bacillus spp. and its Leaching Properties of Slag

Objective: To investigate the growth and the leaching residue of Bacillus spp.Methods: The target species were isolated and identified by 16S rDNA electrophoresis.Traditional method was used to detect their growth characteristics.Leaching properties of three types of slags were analyzed.Results: The spherical form of the microbial plants is flat and the gram stain G+.The organism is movable,solitary,with an optimum growth temperature of 30 ℃,an optimum pH of 3,a facultative type of metabolism and a salinity tol-erance of 4%;The microbial oxidase is negative and catalase positive.By molecular biology analysis,it was identified that this indige-nous microorganism belongs to Bacillus spp.An optimistic result was attained under the conditions of 5 % of microbial inoculum and the medium pH 3: the leaching rate of Cd was 90.4 % in the sediment.The optimum results can be found in the other two slags.Conclusion: Bacillus spp.can be applied in the leaching of Cd in slag.