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| NDRG2 通过影响CD24 调控乳腺癌细胞的粘附能力 | |
| 引用本文： | 郑瑾,刘强 史恒军 任秦有 杨建刚 刘新平 刘文超.NDRG2 通过影响CD24 调控乳腺癌细胞的粘附能力[J].现代生物医学进展,2012,12(1):5-8. |
| 作者姓名： | 郑瑾 刘强 史恒军 任秦有 杨建刚 刘新平 刘文超 |
| 作者单位： | 1. 第四军医大学西京医院肿瘤中心 陕西西安710032;第四军医大学唐都医院中医科暨中西医结合肿瘤科 陕西西安710038 2. 第四军医大学唐都医院血液科 陕西西安710038 3. 第四军医大学唐都医院中医科暨中西医结合肿瘤科 陕西西安710038 4. 生物化学与分子生物学教研室 陕西西安710032 5. 第四军医大学西京医院肿瘤中心 陕西西安710032 |
| 基金项目： | 国家自然科学基金(81072973) |
| 摘 要： | 目的:阐明NDRG2(N-Myc downstream-regulated gene 2)在乳腺癌细胞中对CD24的调控及其对乳腺癌细胞粘附能力的影响。方法:RT-PCR和Western blot方法检测乳腺癌细胞MCF-7及Bcap-37中NDRG2和CD24的表达;通过腺病毒上调MCF-7细胞中NDRG2的表达,或利用siRNA下调Bcap-37细胞中NDRG2的表达,检测CD24基因和蛋白的变化。粘附实验检测改变NDRG2表达水平后对MCF-7及Bcap-37细胞粘附能力的影响。结果:MCF-7细胞中NDRG2基因和蛋白的表达水平低于Bcap-37细胞,而CD24的表达水平高于Bcap-37细胞;在MCF-7细胞中通过腺病毒载体上调NDRG2可以抑制CD24的表达并抑制其粘附能力,而在Bcap-37细胞中利用siRNA下调NDRG2的表达可以提高CD24的水平及细胞的粘附能力;结论:NDRG2通过影响CD24参与调控乳腺癌细胞的粘附能力。 |
| 关 键 词： | NDRG2 CD24 乳腺癌细胞 粘附 |
NDRG2 Expression Regulates CD24 and Adhesion Potential of Breast Cancer Cells |
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| ZHENG Jin,LIU Qiang,SHI Heng-jun,REN Qin-you,YANG Jian-gang,LIU Xin-ping,LIU Wen-chao.NDRG2 Expression Regulates CD24 and Adhesion Potential of Breast Cancer Cells[J].Progress in Modern Biomedicine,2012,12(1):5-8. | |
| Authors: | ZHENG Jin LIU Qiang SHI Heng-jun REN Qin-you YANG Jian-gang LIU Xin-ping LIU Wen-chao |
| Institution: | 1(1 Department of Oncology and State Key Discipline of Cell Biology,Xijing Hospital,The Fourth Military Medical University,Xi’an,710032,China;2 Department of Integrated traditional and western medicine of Oncology,Tangdu Hospital,Xi’an,710038;3 Department of Hematology,Tangdu Hospital,Xi’an,710038;4 Department of Biochemistry and Molecular Biology,the Fourth Military Medical University,Xi’an,710032) |
| Abstract: | Objective: To investigate the effect of N-Myc downstream-regulated gene 2(NDRG2) on the adhesion potential of breast cancer cells.Methods: Gene and protein expressions of NDRG2 and CD24 were analyzed in MCF-7 and Bcap-37 cells.The ex-pression of CD24 and cell adhesion ability was detected after NDRG2 by adenovirus or siRNA treatment.Results: Both the gene and pro-tein expressions of NDRG2 in MCF-7 cells were lower than Bcap-37 cells.In contrast,CD24 expression is higher in MCF-7 cells com-pared to that in Bcap-37 cells.Up-regulated NDRG2 expression leads to the decrease in CD24 expression and cell adhesion ability in MCF-7 cells.In contrast,NDRG2 down-regulation results in enhancing the expression of CD24 and cell adhesion potential in Bcap-37 cells.Conclusion: NDRG2,associated with altered CD24 expression,regulates breast cancer cells adhesion ability. |
| Keywords: | NDRG2 CD24 Breast cancer cell Adhesion |
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