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HMGB1 在a2A- 肾上腺素受体介导脓毒血症大鼠急性肺损伤的作用
引用本文:纪木火,吴晶,田蜜,李仁奇,李国民,范云霞,杨建军.HMGB1 在a2A- 肾上腺素受体介导脓毒血症大鼠急性肺损伤的作用[J].现代生物医学进展,2012,12(2):215-217.
作者姓名:纪木火  吴晶  田蜜  李仁奇  李国民  范云霞  杨建军
作者单位:1. 南京大学医学院南京军区南京总医院麻醉科 江苏南京210002
2. 江苏大学附属金坛医院ICU 江苏金坛213200
基金项目:江苏省"六大人才高峰"课题(2008093);常州市卫生局科技计划项目(WZ201044)
摘    要:目的:探讨HMGB1在a2A-肾上腺素受体介导脓毒血症大鼠急性肺损伤(ALI)的作用。方法:64大鼠建立盲肠结扎穿孔法(CLP)脓毒症模型,随机均分为以下两组:CLP组及CLP+马来酸钠组。各组分别于模型建立后2(T1)、6(T2)、12(T3)、24 h(T4)时检测大鼠血清TNF-、高迁移率族蛋白1(HMGB1)及IL-10含量。CLP24 h后检测肺组织干湿重比(W/D)和髓过氧化物酶活性(MPO)及HMGB1表达;并采用HE法进行肺组织学评分。结果:CLP+马来酸钠组T2时的TNF-水平明显低于CLP组(P<0.05);而HMGB1在T2、T3及T4均明显低于CLP组(P<0.05);IL-10在各个时间点比较结果差异无统计学意义(P>0.05)。CLP+马来酸钠组肺组织W/D、MPO活性、肺组织损伤评分均明显低于CLP组(P<0.05)。CLP+马来酸钠组肺组织HMGB1表达明显低于CLP组(P<0.05)。结论:HMGB1参与了ALI的病理过程,a2A-肾上腺素受体阻断可以通过抑制HMGB1从而改善ALI时的肺功能。

关 键 词:高迁移率族蛋白-1  a2A-肾上腺素受体  脓毒症  急性肺损伤

Effect of High Mobility Group Box-1 Protein on α2A-Adrenoceptors-Mediated Acute Lung Injury in a Rat Model of Sepsis
JI Mu-huo,WU Jing,TIAN Mi,LI Ren-qi,LI Guo-min,FAN Yun-xi,Yang Jian-jun.Effect of High Mobility Group Box-1 Protein on α2A-Adrenoceptors-Mediated Acute Lung Injury in a Rat Model of Sepsis[J].Progress in Modern Biomedicine,2012,12(2):215-217.
Authors:JI Mu-huo  WU Jing  TIAN Mi  LI Ren-qi  LI Guo-min  FAN Yun-xi  Yang Jian-jun
Institution:1(1 Dept.of Anesthesiology,Nanjing General Hospital of Nanjing Military Command,School of Medicine,Nanjing University,Nanjing,210002,China;2 Department of Anesthesiology and Intensive Care,Jintan Hospital,Changzhou,213200,CHINA)
Abstract:Objective: To investigate the effect of high mobility group box-1 protein on α2A-adrenaline receptors-mediated acute lung injury in a rat model of sepsis.Methods: In a sepsis model by cecal ligation and puncture(CLP),sixty-four SD rats were randomly divided into the following groups: CLP group and CLP + maleate group.Plasma levels of TNF-α,HMGB1 and IL-10 were determined at the following time points: 2(T1),6(T2),12(T3) and 24 h(T4) after CLP.Lung was removed for the determinations of pulmonary wet-to-dry ratio(W/D),myeloperoxidase activity(MPO),total histological scores and HMGB1 immunohistochemistry analysis 24 h after CLP.Results: Plasma levels of TNF-α at T2 and HMGB1 at T2,T3 and T4 were significantly lower in CLP + maleate group than CLP group(P<0.05).There was no difference in IL-10 over time between groups(P>0.05).Pulmonary levels of W/D,MPO,total histological scores and HMGB1 expression were significantly lower than those in CLP group(P<0.05).Conclusions: HMGB1 plays a key role in the pathophysiology of ALI,and antagonism of α2A-adrenoceptors improves pulmonary function by inhibiting HMGB1.
Keywords:HMGB1  α2A-adrenaline receptors  Sepsis  Acute lung injury
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