臭椿生物碱canthin-6-one对小鼠镇静催眠作用的研究

摘要 目的：研究臭椿生物碱canthin-6-one对小鼠的镇静催眠作用。方法：选昆明小鼠随机分为空白组、地西泮阳性药物组、canthin-6-one低、中、高给药组（5、10、20 mg?kg^-1），采用阈上剂量和阈下剂量戊巴比妥钠诱导小鼠睡眠，记录入睡小鼠比例、睡眠潜伏期和睡眠持续时间。此外，酶联免疫法测定小鼠脑组织中5-HT、NE、DA和GABA含量。结果：中、高剂量canthin-6-one可使小鼠的站立次数和自主活动次数均明显减少（P＜0.05），可使阈上剂量戊巴比妥钠诱导的小鼠的睡眠潜伏期缩短（P＜0.05）；各剂量组可使阈下剂量戊巴比妥钠诱导的小鼠入睡率提高（P＜0.05）。此外，中、高剂量canthin-6-one也可降低小鼠脑组织中5-HT和NE含量，增加GABA含量（P＜0.05），对DA的含量有影响，但无统计学意义（P＞0.05）。结论：Canthin-6-one有明显的协同戊巴比妥钠改善睡眠作用，其作用与相关神经递质（5-HT、NE、GABA）具有密切关系。

The Effects of Alkaloid Canthin-6-one from Ailanthus Altissima on Sedation and Hypnosis in Mice

ABSTRACT Objective: To discuss the effects of alkaloid canthin-6-one from Ailanthus altissima on sedation and hypnosis in mice. Methods: Kunming mice were randomly divided into a blank group, a diazepam-positive drug group, and a low, medium and high dose groups of canthin-6-one (5, 10, 20 mg?kg^-1). Then, the mice were induced to sleep using pentobarbital sodium with suprathreshold and subthreshold dose by intraperitoneal injection. The proportion of sleeping, sleep latency and sleep duration of mice were recorded. The contents of 5-HT, NE, DA and GABA of brain tissue were measured by enzyme linked immunosorbent assay according to the manufacturer''s instruction. Results: The duration of spontaneous activity and standing was significantly decreased (P<0.05), the sleep latency and the sleep time with the suprathreshold dose of pentobarbital sodium were also decreased (P<0.05). The proportion of sleeping mice with the subthreshold dose of pentobarbital sodium was significantly improved by-injected with low, medium and high dose of canthin-6-one (P<0.05). Moreover, the content of 5-HT, NE in brain tissues were markedly reduced, while the content of GABA was markedly increased by medium and high dose of canthin-6-one (P<0.05). The content of DA in brain tissues were influenced by canthin-6-one, but it is not statistically significant (P>0.05). Conclusion: Canthin-6-one had obvious synergistic effects on improving sleeping quality combined with pentobarbital sodium. The mechanism is correlated to neurotransmitter (5-HT, NE, GABA) release in the brain.