结直肠癌患者血浆甲基化Sept9基因的表达及临床意义

目的:探讨甲基化Sept9基因在结直肠癌患者血浆中的表达及临床意义。方法:选择2015年1月~2017年3月经陕西省人民医院病理证实的结直肠癌患者87例(结直肠癌组)、结直肠息肉患者79例(结直肠息肉组)、健康体检者93例(健康对照组)作为研究对象,采用实时荧光定量聚合酶链式反应(PCR)技术检测其外周血血浆Sept9基因甲基化情况,比较三组甲基化Sept9基因阳性表达率,分析甲基化Sept9基因阳性表达与结直肠癌病理特征的关系。结果:血浆甲基化Sept9基因在结直肠癌组、结直肠息肉组、健康对照组的阳性表达率分别为71.26%(62/87)、5.06%(4/79)、3.23%(3/93),差异有统计学意义(P0.05);血浆甲基化Sept9基因阳性表达与结直肠癌患者的性别、年龄、肿瘤部位、病理分型、血管侵犯、神经侵犯无关(P0.05),与肿瘤最大径、浸润深度、分化程度、淋巴结转移、TNM分期有关(P0.05);结直肠癌患者血浆甲基化Sept9基因阳性表达率为71.26%(62/87),高于血清CEA的54.02%(47/87)、CA199的35.63%(31/87)、CA724的33.33%(29/87)、CA125的21.84%(19/87),差异有统计学意义(P0.05)。结论:结直肠癌患者外周血血浆甲基化Sept9基因呈高表达状态,早期检测甲基化Sept9基因表达水平在结直肠癌的诊断及病情评估中有重要意义。

Expression and Clinical Significance of Plasma Methylated Sept9 Gene in Patients with Colorectal Cancer

ABSTRACT Objective: To explore the expression and clinical significance of plasma methylated Sept9 gene in patients with col- orectal cancer. Methods: A total of 87 patients with colorectal cancer (colorectal cancer group), 79 patients with colorectal polyps (col- orectal polyps group), who were confirmed by pathology in shanxi province people''s hospital from January 2015 to March 2017, and 93 healthy volunteers (healthy control group) were chosen as subjects. The Quantitative Real-time Polymerase Chain Reaction (PCR) tech- nique was used to detected the methylated of Sept9 gene in peripheral blood plasma. The positive expression rates of methylated Sept9 gene in the three groups were compared. The relationship between positive expression of methylated Sept9 gene and pathological fea- tures of colorectal carcinoma was analyzed. Results: The positive expression rates of plasma methylated Sept9 gene in the colorectal can- cer group, the colorectal polyps group and the healthy control group were 71.26% (62/87), 5.06% (4/79) and 3.23% (3/93) respectively, the differences were statistically significant (P<0.05). The positive expression of plasma methylated Sept9 gene was not related to the gender, age, tumor location, pathological type, vascular invasion and nerve invasion in the patients with colorectal cancer (P>0.05), but related to the maximum tumor diameter, infiltrating depth, differentiation degree, lymphatic metastasi and TNM staging (P<0.05). The positive expression rate71.26%(62/87))] of plasma methylated Sept9 gene in the patients with colorectal cancer were higher than that 54.02% (47/87) of serum CEA, 35.63% (31/87) of CA199, 33.33% (29/87) of CA724 and 21.84% (19/87) of CA125, the differences were statistically significant (P<0.05). Conclusion: The plasma methylated Sept9 gene is highly expressed in the patients with colorectal cancer, so early detection of the expression level of the methylated Sept9 gene has important significance in the early diagnosis and as- sessment of colorectal cancer.