miR-125a-3p调控人结肠癌细胞浸润转移的作用及机制研究

目的:探讨miR-125a-3p在结肠癌细胞浸润与转移中的作用及其可能机制。方法:通过qRT-PCR方法检测miR-125a-3p在结肠癌细胞及组织样本中的表达;在结肠癌细胞过表达或沉默miR-125a-3p后,通过平板克隆实验、MTT实验、划痕实验、Transwell实验检测结肠癌细胞增殖、迁移及侵袭能力的变化;采用Western blot方法检测miR-125a-3p过表达后相关标志分子的表达水平变化情况。结果:miR-125a-3p在结肠癌细胞及组织呈现异常低表达;过表达miR-125a-3p抑制结肠癌细胞HCT116及SW480的增殖能力;过表达或沉默miR-125a-3p分别抑制或增强结肠癌细胞的迁移与侵袭能力;过表达miR-125a-3p在mRNA及蛋白水平均能够显著抑制Snail、N-cadherin及Vimentin的表达,而增加E-cadherin的表达。结论:miR-125a-3p参与调节结肠癌细胞浸润与转移,其机制可能是通过调控上皮间质转化途径介导的。

Effect and Mechanism of miR-125a-3p on the Infiltration and Metastasis of Human Colon Cancer Cells

ABSTRACT Objective: To explore the effect and mechanism of miR-125a-3p on the infiltration and metastasis of colon cancer cells. Methods: The expression of miR-125a-3p in colon cancer cells and tissue samples were detected by qRT-PCR. The miR-125a-3p expression in the colon cancer cells was overexpressed or down-regulated, then the effects of miR-125a-3p on the proliferation, migration and invasion of colon cancer cells were detected by colony formation assay, cell proliferation assay, wound healing assay and transwell arrays. The expressions of epithelial-mesenchymal transition(EMT) related markers were detected by Western blot after miR-125a-3p-overexpressed colon cancer cells. Results: The expression of miR-125a-3p was significantly down-regulated in both colon cancer cells and tissues. Overexpression of miR-125a-3p inhibited the proliferation of colon cancer cells HCT116 and SW480. Overex- pression or silence of miR-125a-3p inhibited or enhanced the migration and invasion of colon cancer cells respectively. In miR-125a-3p-overexpressed colon cancer cells, the expression of the Snail, N-cadherin and Vimentin were significantly decreased, whereas E-cadherin expression was increased in both mRNA and protein levels. Conclusion: miR-125a-3p participated in regulating the migration and invasion of colon cancer cells. The mechanism may be mediated by regulating the EMT pathway.