高海拔地区藏族妇女P21Ser31Arg多态性及与宫颈癌遗传易感性的相关性研究

目的:研究高海拔地区藏族妇女宫颈组织中P21Ser31Arg的多态性,并分析其与宫颈癌遗传易感性的相关性。方法:选择2015年7月~2016年6月收治的200例宫颈癌患者、100例宫颈上皮内瘤样病变(CIN)患者、100例宫颈炎患者及100例正常宫颈患者为研究对象,采用限制性酶切片段长度多态性分析(PCR-RFLP)方法检测各组患者宫颈组织中P21Ser31Arg三种基因型Ser/Ser、Ser/Arg及Arg/Arg的分布情况并比较其构成差异,进一步分析其多态性与宫颈癌患者年龄、癌症分型及分化程度的相关性。结果:四组患者宫颈组织中均检测到三种基因型,Ser/Arg、Ser/Ser及Arg/Arg三种基因型的构成比分别为53.38%、34.88%及11.75%。四组患者宫颈组织中P21Ser31Arg的三种基因型分布整体比较及各组两两比较,差异均无统计学意义(P0.05)。200例宫颈癌患者中,不同年龄组及不同分型组患者的P21Ser31Arg基因型分布比较,差异无统计学意义(P0.05);而不同分化程度组浸润性鳞癌患者的三种基因型分布比较,差异具有统计学意义(P0.05)。结论:未发现P21Ser31Arg多态性与高海拔地区藏族妇女宫颈癌的遗传易感性有关,而可能与宫颈癌患者的分化程度相关。

Association Between P21Ser31Arg Polymorphism and Genetic Susceptibility to Cervical Cancer in Tibetan Women at High Altitude

ABSTRACT Objective: To study the polymorphism of P21Ser31Arg in cervical tissues of Tibetan women at high altitude, and to analyze its correlation with the genetic susceptibility to cervical cancer. Methods: All the objects were selected from the patients during July 2015 to June 2016, including 200 cases of patients with cervical cancer, 100 cases of patients with cervical intraepithelial neoplasia (CIN), 100 cases of patients with cervicitis and 100 cases of patients with normal cervical. The distribution of the three genotypes (Ser/Ser, Ser/Arg and Arg/Arg) of P21Ser31Arg in cervical tissues of all groups were detected by the method of restriction fragment length polymorphism (PCR-RFLP), and the the composition differences in different groups were compared. The association of polymorphism with age, type of cancer and differentiation of cervical cancer was further analyzed. Results: Three genotypes of P21Ser31Arg in cervical tissues of the four groups were all detected, and the constituent ratio of Ser/Arg, Ser/Ser and Arg/Arg were respectively 53.38%, 34.88% and 11.75%. There was no significant difference of the distribution of three genotypes of P21Ser31Arg among the four groups and between each two groups (P>0.05). In the 200 cases of cervical cancer patients, there was no significant difference the distribution of P21Ser31Arg genotypes in different groups with various ages and types of cancer (P>0.05); and the difference of distribution of three genotypes in the groups with different differentiation was statistically significant (P<0.05). Conclusion: P21Ser31Arg polymorphism may not associated with genetic susceptibility to cervical cancer in Tibetan women at high altitude, but may be related to the degree of differentiation in cervical cancer patients.