子宫内膜卵巢双癌组织中SOX2和OCT4的表达及意义

目的:探讨SOX2和OCT4蛋白在宫内膜样子宫和卵巢双发恶性肿瘤(double endometrioid endometrial and ovarian carcinomas,DEEOC)中的表达情况及意义。方法:收集青岛大学附属医院2007年-2016年30例DEEOC石蜡组织标本,采用免疫组化法检测SOX2和OCT4的表达,分析DEEOC两部位癌组织中及原发性、转移性DEEOC癌组织中SOX2和OCT4蛋白表达差异及相关性。结果:SOX2和OCT4在DEEOC两部位癌组织中的表达率明显高于相应的正常组织(P均0.001),SOX2在原发性DEEOC、转移性DEEOC两部位癌组织中表达均相当(P均0.05),OCT4在原发性DEEOC、转移性DEEOC中的表达也相当(P均0.05),且Pearson相关性分析显示双癌组织中的两种蛋白的表达均呈正相关性。转移性双癌两部位组织中的SOX2和OCT4的表达量都要明显高于原发性双癌(P均0.05)。结论:DEEOC癌组织中SOX2和OCT4均呈阳性表达,二者可能相互作用参与DEEOC肿瘤的发生、发展,在辅助区分原发性和转移性DEEOC也可能具有一定的指导意义。

Expression and Significance of OCT4 and SOX2 in the Simultaneous Endometrial and Ovarian Endometrioid Carcinomas

ABSTRACT Objective: To study the protein expression of SOX2 and OCT4 in double endometrioid endometrial and ovarian carcinomas (DEEOC) and the clinical significance. Methods: 30 cases of DEEOC patients were enrolled from the Pathology department of the Affiliated Hospital of Qingdao University from year 2007 to 2016. Immunohistochemical staining was used to measure the expressions of SOX2 and OCT4. Results: Both SOX2 and OCT4 were over-expressed in the two cancer sites of DEEOC compared to normal endometrial tissues and normal ovarian tissues (P<0.001). There was no significant difference in the SOX2 expression between double cancer tissues in primary DEEOC samples (P = 0.307) and metastatic DEEOC (P=0.647). Additionally, no significant difference was observed in the OCT4 expression between the two tissues primary DEEOC samples (P=0.136) and metastatic DEEOC(P=0.653). A correlation between SOX2 and OCT4 expression in all DEEOC cancer tissues were found. Besides, both SOX2 and OCT4 expressions were higher in metastatic DEEOC tissues compared to corresponding primary DEEOC tissues (both P<0.05). Conclusion: Both SOX2 and OCT4 were positively expressed in DEEOC tissues. Moreover, SOX2 and OCT4 may play similar roles in the tumorigenesis and progression of DEEOC, which may have some implication in distinguishing the primary DEEOC and metastatic DEEOC.