白藜芦醇抑制小鼠肥胖的功效及相关机制探讨

目的:研究白藜芦醇抑制高脂引起的肥胖的作用机制。方法:将18只C57小鼠随机分为3组,分别为对照组、高脂以及高脂+白藜芦醇小鼠模型,给小鼠喂养一定剂量白藜芦醇(100 mg/kg/d),喂养12周。提取小鼠皮下脂肪细胞,分化成熟,加入白藜芦醇,采用q RT-PCR以及Western blot等方法检测HO-1以及棕色脂肪标志基因的表达。通过q RT-PCR检测小鼠脂肪组织炎症因子、UCP-1以及HO-1的表达。结果:白藜芦醇在体内可以明显抑制高脂引起的肥胖,糖耐量异常,同时促进棕色脂肪标志基因UCP-1,PGC-1以及PRDM16的表达。白藜芦醇还可抑制肥胖小鼠脂肪组织炎症因子的增加以及抗炎蛋白HO-1的表达。在体外分化的成熟的皮下脂肪细胞中,白藜芦醇同样可以促进棕色脂肪标志基因UCP-1,PGC-1以及PRDM16的表达。白藜芦醇通过促进抗炎蛋白HO-1的表达抑制高脂引起的脂肪炎症反应。结论:白藜芦醇可以通过促进白色脂肪棕色化以及抑制慢性低度炎症抑制高脂引起的肥胖、糖耐量异常以及改善胰岛素敏感性。

Resveratrol Exerts Anti-obesity Effect Involving Modulation of UCP-1 and HO-1

ABSTRACT Objective: Investigated the mechanisms underlying resveratrol inhibited HFD-induced obesity. Methods: In this study we investigated the anti-obesity effect of resveratrol in vivo and in vitro. Four-week-old Mice were fed with normal diet (ND, n=6), high-fat diet (60% energy from fat, HFD, n=6), high-fat diet with resveratrol daily at a dose of 100 mg/kg/day (RSV, n=6), for 12 weeks. We also detected the effects of resveratrol on brown adipocytes markers and HO-1 expression in primary subcutaneous adipocytes. Then, expressions levels of HO-1 and brown adipocytes marks were analyzed by qPCR and Western blot. Results: The results showed that resveratrol significantly inhibited mice body weight induced by high fat diet. In this study, we report the identification of resveratrol, as a novel inducer of brown adipoctyes genes UCP-1, PGC-1 and PRDM16 expression in vivo. Additionally, resveratrol also inhibited inflam- matory mediators and induced anti-inflammatory enzyme HO-1 expression in adipose tissue. Further, in primary subcutaneous adipocytes, resveratrol also induced brown adipocytes marker genes expression including UCP-1, PGC-1 and PRDM16. The anti-inflam- matory effects of resveratrol dependent on HO-1 expression in vitro. Conclusion: Altogether, our results demonstrate that resveratrol in- duces brown adipocytes marker genes and anti-inflammatory gene HO-1 expression, thereby inhibits high fat diet-induced obesity and in- sulin resistance.