| FMR-1基因中CGG重复序列扩增条件的优化 |
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| 引用本文： | 马云,何淑雅,Nanbert Zhong,李斌元,喻长顺,苏娇.FMR-1基因中CGG重复序列扩增条件的优化[J].现代生物医学进展,2007,7(1):12-14. |
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| 作者姓名： | 马云 何淑雅 Nanbert Zhong 李斌元 喻长顺 苏娇 |
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| 作者单位： | 1. 南华大学生物化学与分子生物学教研室,衡阳,421001
2. 美国纽约州立基础研究所人类遗传研究室 |
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| 基金项目： | 国家自然科学基金;湖南省教育厅科研项目;衡阳市科技局课题 |
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| 摘 要： | 目的:优化PCR扩增条件,建立一种有效检测脆性X综合征的方法。方法:在常规PCR的基础上,采用耐高温酶替代法、碱基替代法,同时加入有机溶剂DMSO等,对表型正常的人群进行FMR1基因CGG重复序列检测。结果:改良PCR法可以提高G C富集区扩增效率,并取得了较好的效果。结论:建立了一种扩增FMR-1基因中CGG重复序列的可行方法。
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| 关 键 词： | FMR-1基因 CGG重复序列 |
| 文章编号： | 24081114 |
| 修稿时间： | 10 13 2006 12:00AM |
Optimization of PCR Conditions of CGG Repeat Sequence in FMR-1 Gene |
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| Nanbert Zhong.Optimization of PCR Conditions of CGG Repeat Sequence in FMR-1 Gene[J].Progress in Modern Biomedicine,2007,7(1):12-14. |
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| Authors: | Nanbert Zhong |
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| Institution: | 1. Institute of Biochemistry and Molecular Biology, Nanhua University; 2. SCL-Molecular Neurogenetic Diagnostic Laboratory, NY, America |
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| Abstract: | Objective:To establish an efficient method to detect fragile X chromosome syndrome(FraX)by optimizing PCR amplifying condition.Methods:On the basis of conventional PCR,the CGG repeat sequence in FMR-1 gene of the crowd with normal phenotype was detected by hyper-thermoresistant enzyme-replacement,base-replacement and adding an organic sol- vent DMSO at the same time.Results:Improved PCR could increase the efficiency of amplification in G and C enrichment re- gions,and obtain better effect.Conclusion:A feasible method of amplifying the CGG repeat sequences in FMR-1 gene was estab- lisbed. |
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| Keywords: | PCR |
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