氧化钛纳米管对即刻种植骨结合影响的实验研究

目的:评价氧化钛纳米管对犬即刻种植骨结合效果的影响。方法:犬拔牙后即刻将光滑表面(对照组)和氧化钛纳米管表面(实验组)种植体植入拔牙窝内,于12周后处死取材,进行显微CT扫描、组织学染色分析以及生物力学检测。结果:扫描电镜显示经过阳极氧化后,钛表面形成了直径为30-80纳米的纳米管状结构;12周后,显微CT扫描结果提示实验组骨体积分数(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)均显著高于对照组,骨小梁间隙(Tb.Sp)显著低于对照组,差异具有统计学意义(P0.05)。术后12周,实验组与对照组骨结合率分别为49.35±11.76%、31.79±13.07%,最大拔出力分别为105.28±27.87N、79.23±20.46N,实验组均显著高于对照组,差异均具有统计学意义(P0.05)。结论:氧化钛纳米管表面有利于促进即刻种植后骨结合的效果。

Effect of TiO2 Nanotubes Surface on Osseointegration in Implantation Immediately after Tooth Extraction

Objective:The aim of this study was to evaluate the effect of TiO2 nanotube surface on osseointegration in implantation immediately after tooth extraction.Methods:Implant with smooth and nanotube surface were inserted into fresh tooth socket of beagle dogs immediately after premolars were extracted. At 12 weeks after implantation, all the animals were sacrificed for micro-CT scanning, histological analysis and biochemical test.Results:At 12 weeks after implantation, Micro-CT shown that bone volume ratio (BV/TV), trabecular number (Tb.N) and trabecular thickness (Tb.Th) increased significantly in the nanotube suface, and compared to smooth surface, the trabecular space (Tb.Sp) of nanotube surface decreased significantly. The bone implant contact (BIC) was 49.35± 11.76%, 31.79± 13.07% in nanotube surface and smooth surface respectively, and the maximum pull out force of nanotube and smooth surface were 105.28 ± 27.87N and 79.23 ± 20.46N.Conclusion:Implant with nanotube surface fabricated with anodization could improve osseointegration in implantation immediately after tooth extraction.