双向凝胶电泳在先天性肛门直肠畸形中的初步应用 |
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引用本文: | 王建尧,刘磊,姚君,叶明,王斌,叶晓硕,冯奇,宋雪倩,邹沙芹,朱思聪.双向凝胶电泳在先天性肛门直肠畸形中的初步应用[J].现代生物医学进展,2013(32):6258-6260,6275. |
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作者姓名: | 王建尧 刘磊 姚君 叶明 王斌 叶晓硕 冯奇 宋雪倩 邹沙芹 朱思聪 |
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作者单位: | [1]深圳市儿童医院肝胆肿瘤外科,广东深圳518000 [2]暨南大学第二临床学院,广东深圳518026 |
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基金项目: | 深圳市科技计划项目(201103154) |
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摘 要: | 目的:寻找先天性肛门直肠畸形患儿直肠末端组织中异常表达的蛋白质。方法:通过二维凝胶电泳分离先天性肛门直肠畸形患儿直肠末端组织及正常新生儿直肠末端组织,用Image Master2D Platium6.0软件比较电泳图谱中的异常蛋白质点。结果:筛选出19个表达差异的蛋白质点,其中有12个蛋白质点表达上调,7个蛋白质点表达下调,差异具有统计学意义。结论:先天性肛门直肠畸形可以导致血清中多种蛋白的异常表达。这些差异表达的蛋白可以为先天性肛门直肠畸形的进一步研究提供了依据。
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关 键 词: | 新生儿 先天性肛门直肠畸形 蛋白组学 二维凝胶电泳 |
Proteome Analysis of Anorectal malformations by Two-dimensional Electrophoresis |
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Institution: | WANG Jian-yao, LIU Lei, YAO Jun, YE Ming, WANG Bin, YE Xiao-shuo, FENG Qi, SONG Xue-qian, (1 Department of Hepatobiliary Surgery, Children's Hospital of Shenzhen, Shenzhen, Guangdong,518000, China; 2 The Second APlfliated Medical School of the Jinan University, Shenzhen, Guangdong, 518026, China) |
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Abstract: | Objective: To search for differentially expressed serum proteins in anorectal malformations (ARM). Methods: Serum proteins from ARM and the normal babies were separated using two-dimensional (2-D) gel electrophoresis. Differentially proteins spots on the 2-I) maps were selected and further analyzed using Image Master2D Platium 6.0 sottware. Results: 19 differentially expressed protein spots were found, among which 12 proteins were evaluated, 7 proteins were down regulated. Conclusion: Good reproducibility and resolution could be obtained in the tissues of ARM by applying immobilized pH gradient 2-dimensional polyacrylamide gel electrophoresis. Screened differentially expressed proteins may provide the candidates of the markers for early detection of ARM. |
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Keywords: | Neonates ARM Dimensional gel electrophoresis |
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