脑挫裂伤致miR-9 升高促进神经干细胞分化

目的:探讨颅脑损伤后miR-9表达的变化和对神经干细胞分化和增值的影响,为颅脑损伤后神经功能修复治疗提出新的思路。方法:通过RT-PCR技术检测miR-9在挫裂伤脑组织中的表达情况;培养胚胎来源神经干细胞,并通过免疫荧光鉴定神经干细胞及其分化;转染miR-9后,通过MTT测定神经干细胞的增殖情况,和流式细胞仪检测分化神经元所占比例。结果:miR-9在挫裂伤脑组织中表达显著上升。对神经干细胞过表达miR-9可显著促进细胞增殖,并诱导分化成神经元。结论:脑挫裂伤时miR-9显著升高,并具有着促进神经干细胞增值和诱导分化的作用,可为伤后神经功能修复提供新的治疗方法。

Up-regulation of miR-9 Regulates Adult Neural Stem Cell Proliferation and Differentiation in Traumatic Brain Injury

Objective:To investigate the expression of miR-9 in traumatic brain injury and the impact of miR-9 for proliferation and differentiation of neural stem cells;to propose new ideas for neurological functions repair in traumatic brain injury.Methods:The expression of miR-9 was detected by RT-PCR in traumatic brain tissues;the embryonic neural stem cells were cultured and identified by immunofluorescence;After miR-9 transfection,the proliferation of neural stem cells were measured by MTT,and the proportion of d...