| 细胞周期蛋白依赖激酶Roscovitine 诱导非小细胞肺癌A549 细胞凋亡及
其机制的研究 |
| |
| 引用本文： | 张海华,孟瑾,张峰.细胞周期蛋白依赖激酶Roscovitine 诱导非小细胞肺癌A549 细胞凋亡及
其机制的研究[J].现代生物医学进展,2011,11(20):3835-3837. |
| |
| 作者姓名： | 张海华 孟瑾 张峰 |
| |
| 作者单位： | 1. 第四军医大学口腔医学系 陕西 西安710032
2. 第四军医大学药学院药理学教研室 陕西 西安710032 |
| |
| 摘 要： | 目的:探讨细胞周期蛋白依赖激酶(CDK)抑制剂Roscovitine(Ros)诱导非小细胞肺癌(NSCLC)A549细胞凋亡及其作用机制。方法:以不同浓度Ros(10μM、20μM、40μM)处理细胞24h,采用Annexin V-PI染色以流式细胞仪检测细胞凋亡,Westernblot法检测胞浆中和线粒体促凋亡蛋白Bax和Bad的表达,流式细胞仪检测线粒体膜电位(MMP)变化。结果:Ros以剂量依赖的方式诱导A549细胞凋亡,同时Bad和Bax在胞浆的含量随着Ros剂量的增加而减少,而在线粒体中却出现相反的结果,线粒体膜电位随Ros剂量的增大而降低。结论:Ros可通过促进Bax和Bad由胞浆向线粒体易位,诱导NSCLC A549细胞由线粒体途径发生凋亡。
|
| 关 键 词： | Roscovitine 肺癌细胞 细胞凋亡 Bax Bad |
Cyclin Dependent Kinase Inhibitor Roscovitine-induced Apoptosis in
Non-small Cell Lung Cancer A549 Cells and Its Mechanisms |
| |
| ZHANG Hai-hu,MENG Jin,ZHANG Feng.Cyclin Dependent Kinase Inhibitor Roscovitine-induced Apoptosis in
Non-small Cell Lung Cancer A549 Cells and Its Mechanisms[J].Progress in Modern Biomedicine,2011,11(20):3835-3837. |
| |
| Authors: | ZHANG Hai-hu MENG Jin ZHANG Feng |
| |
| Institution: | ZHANG Hai-hua1,MENG Jin2,ZHANG Feng2(1School of Clinical Medicine and Stomatology,2Department of Pharmacology,School of Pharmacy,The Fourth Military Medical University,Xi'an,710032,China) |
| |
| Abstract: | Objective:To investigate the effect of cyclin dependent kinase inhibitor roscovitine(Ros)-induced apoptosis on non-small cell lung cancer A549 cells and its mechanisms.Methods:A549 cells were exposed to varying concentrations of Ros for 24h.Apoptosis was detected with Annexin V-PI staining by flow cytometry.The expression of pro-apoptotic protein Bax and Bad in cytoplasm and mitochondria was detected by western blot.The changes of mitochondrial membrane potential(MMP) were analyzed by flow cytometry.Results... |
| |
| Keywords: | Roscovitine Non-small cell lung cancer Apoptosis Bax Bad |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《现代生物医学进展》浏览原始摘要信息 |
| 点击此处可从《现代生物医学进展》下载免费的PDF全文 |
