重组标签蛋白在蛋白质纯化中的研究进展

重组蛋白的表达纯化是研究蛋白质结构与功能的重要环节之一,表达重组蛋白宿主细胞的监测筛选和重组蛋白低水平可溶性表达、低回收率以及不稳定易水解等问题一直是蛋白质高通量纯化工艺中的难题。近年来,将多肽或蛋白质作为标签,与目标蛋白共表达的方法已经很大程度地解决了这一问题。因此,针对不同特性的蛋白质选择合适的标签纯化策略在重组蛋白纯化研究中是相当关键的环节。本文回顾了几种传统标签蛋白的研究概况(His-tag,Arg-tag,Flag-tag,GST-tag,MBP-tag等),着重对近年来新开发的了几种标签蛋白(Si-tag,Halo-tag,Intein-CBD-tag,ELPs-tag等)进行了深入探讨,并对新标签蛋白在蛋白质纯化中的应用前景作以展望。

Progress of Novel Tag Protein Fusions for the Recombinant Protein Purification

Recombinant protein expression and purification is one of the vital sections for structure determination and function study of proteins. Detection and selection of host strain containing target protein, low-yield recombinant protein expression by insolubility, low-recovery ratio, instability and proteolysis etc., which always lead to difficulties in high-throughput protein purification processes. Recently, the co-expression methods of target protein fused polypeptides or protein as tag have successfully overcome this problemto a large extent. Therefore, selecting appropriate tag of protein purification for various property of proteins is the key portion. In this paper, the progresses on several traditional tag proteins (His-tag, Arg-tag, Flag-tag, GST-tag, MBP-tag etc.) are reviewed. Moreover, several recently developed tag proteins (Si-tag, Halo-tag, intein-CBD-tag, ELPs-tag etc.) and the prospective in this field are also discussed.