| 细胞内铜/锌超氧化物歧化酶在人胸主动脉夹层的表达研究 |
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| 引用本文： | 杨琳,廖明芳,邹思力,侯乐伟,翁剑锋,季欣然,黄娟,刘德鼎,刘延玲,景在平.细胞内铜/锌超氧化物歧化酶在人胸主动脉夹层的表达研究[J].现代生物医学进展,2010,10(3). |
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| 作者姓名： | 杨琳 廖明芳 邹思力 侯乐伟 翁剑锋 季欣然 黄娟 刘德鼎 刘延玲 景在平 |
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| 作者单位： | 第二军医大学附属长海医院,血管外科,上海,200433 |
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| 基金项目： | 国家自然科学基金(30600599); 上海市科委基础重点项目(08JC1406100) |
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| 摘 要： | 目的:探讨细胞内铜/锌超氧化物岐化酶(copper zinc superoxide dismutase,Cu/Zn-SOD,SOD-1)在人胸主动脉夹层(humanthoracic aortic dissection,hTAD)中的表达情况及其在hTAD中的可能作用。方法:蛋白质印迹法(Western blot,WB)检测SOD-1在TAD和正常人胸主动脉(NA)中膜组织中的表达情况,免疫组织化学染色(immunohistochemistry,IHC)验证SOD-1在动脉壁中的表达和定位。结果:蛋白质印迹和免疫组化染色均显示SOD-1在TAD组表达量较NA组减低(P<0.05);免疫组化染色进一步显示,SOD-1主要位于主动脉壁中膜平滑肌细胞的胞质内,其在夹层主动脉壁中膜撕开处表达缺失。结论:SOD-1在TAD中表达量减少,可能由于参与氧化应激引起的脂质过氧化和炎症反应,以及细胞外基质(extracellular matrix,ECM)的降解等机制所致。
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| 关 键 词： | 细胞内铜/锌超氧化物岐化酶(SOD-1) 胸主动脉夹层(TAD) 氧化应激 脂质过氧化 细胞外基质 |
Expression of intracellular copper zinc superoxide dismutase in human thoracic aortic dissection |
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| YANG Lin,LIAO Ming-fang,ZOU Si-li,HOU Le-wei,WENG Jian-feng,JI Xin-ran,HUANG Juan,LIU De-ding,LIU Yan-ling,JING Zai-ping.Expression of intracellular copper zinc superoxide dismutase in human thoracic aortic dissection[J].Progress in Modern Biomedicine,2010,10(3). |
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| Authors: | YANG Lin LIAO Ming-fang ZOU Si-li HOU Le-wei WENG Jian-feng JI Xin-ran HUANG Juan LIU De-ding LIU Yan-ling JING Zai-ping |
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| Institution: | YANG Lin,LIAO Ming-fang,ZOU Si-li,HOU Le-wei,WENG Jian-feng,JI Xin-ran,HUANG Juan,LIU De-ding,LIU Yan-ling,JING Zai-ping (Department of Vascular Surgery,Changhai Hospital,the Second Military Medical University,Shanghai 200433 China) |
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| Abstract: | Objective:To investigate the intracellular copper zinc superoxide dismutase (SOD-1) expression in human thoracic aortic dissection (hTAD) and its possible roles in TAD.Methods:Western blot was performed to detect the expression of SOD-1 in dissected thoracic aortas and normal controls.Immunohistochemistry was used to validate its expression and localization. Results: Western blot and immunohistochemistry showed that the SOD-1 expression decreased in TAD compared with normaI controls (P< 0.05).Immunohistoche... |
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| Keywords: | Copper zinc superoxide dismutase (SOD-1) Thoracic aortic dissection (TAD) oxidative stress lipid peroxidation extracellular matrix(ECM) |
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