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| 慢病毒靶向携带Survivin siRNA抗肺腺癌的体内研究 | |
| 引用本文： | 赵岐刚,司磊,田小侠,朱桂锋,王笑峰,刘霞.慢病毒靶向携带Survivin siRNA抗肺腺癌的体内研究[J].现代生物医学进展,2010,10(2). |
| 作者姓名： | 赵岐刚 司磊 田小侠 朱桂锋 王笑峰 刘霞 |
| 作者单位： | 1. 青岛大学医学院,山东,青岛,266021;山东省聊城市人民医院,山东,聊城,252000 2. 山东省聊城市人民医院,山东,聊城,252000 3. 青岛大学医学院,山东,青岛,266021 |
| 基金项目： | 山东省聊城市科技攻关计划资助项目(20070248) |
| 摘 要： | 目的:探讨慢病毒载体介导的靶向survivin基因小干涉RNA(survivin-siRNA)对裸鼠移植人肺腺癌的体内抑瘤活性。方法:构建表达survivin-siRNA的慢病毒载体和移植人肺腺癌裸鼠模型,肿瘤组织局部注射survivin-siRNA慢病毒载体,观察肿瘤体积及随时间生长变化;PI染色检测细胞凋亡;流式细胞术检测肿瘤细胞周期变化。结果:慢病毒载体介导survivin-siRNA对裸鼠肺腺癌的抑瘤率为46.07%;30～35%的肿瘤细胞凋亡;G1期细胞比例明显增加,S期细胞比例则明显减少。结论:靶向survivin的RNAi能有效抑制裸鼠移植人肺腺癌的生长,诱导肿瘤细胞凋亡和细胞周期改变。 |
| 关 键 词： | RNAi survivin 慢病毒载体 肺腺癌 |
Study on lentivirus targeted to bring Survivin siRNA in anti-lung adenocarcinoma in vivo |
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| ZHAO Qi-gang,Si Lei,TIAN Xiao-xi,ZHU Gui-feng,WANG Xiao-feng,LIU Xia.Study on lentivirus targeted to bring Survivin siRNA in anti-lung adenocarcinoma in vivo[J].Progress in Modern Biomedicine,2010,10(2). | |
| Authors: | ZHAO Qi-gang Si Lei TIAN Xiao-xi ZHU Gui-feng WANG Xiao-feng LIU Xia |
| Institution: | ZHAO Qi-gang1,2,Si Lei2,TIAN Xiao-xi2,ZHU Gui-feng2,WANG Xiao-feng1,LIU Xia1(1 Mdical College of Qingdao University,Qingdao 266021,China1,2 The Lliaocheng People\'s Hospital Sh,ong 252000 ) |
| Abstract: | Objective:To investigate tumor inhibition effect of lentiviral vectors siRNA for survivin to human lung adenocarcinoma. Methods:The lentiviral vectors that survivin-siRNA and the human lung adenocarcinoma-bearing nude mice model were constructed. The lentiviral vectors were local injected in tumor tissue and the change of tumor volume at different time were detected. Decteded cell apoptosis by PI. The change of cell cycle were detected by FCM. Results:The tumor inhibition rate was 46.07%,it was significant ... |
| Keywords: | RNAi Survivin lentiviral vector lung adenocarcinoma |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |