灵孢多糖辅助治疗抑郁症的作用及机制

目的:建立大鼠抑郁模型,研究和探讨灵孢多糖注射液辅助治疗抑郁症的作用及机制。方法:将48只SD大鼠随机分为正常组、抑郁组、生理盐水组及灵孢多糖(低剂量、中剂量、高剂量)治疗组各8只。应用慢性应激刺激的方法诱导抑郁组、生理盐水组、灵孢多糖治疗组大鼠抑郁,通过行为学指标评估建模是否成功。建模成功后,在生理盐水组中注射生理盐水;在灵孢多糖治疗组中分别注射低、中、高剂量灵孢多糖注射液,比较各组间的疗效差异。采用免疫组化的方法测定大鼠脑内多巴胺、去甲肾上腺素、5-羟色胺、酪氨酸羟化酶等单胺类神经递质含量的表达情况。结果:成功的建立了大鼠抑郁模型,抑郁组中大鼠各项行为学指标与正常组相比均明显减少,差异有统计学意义(P0.05);灵孢多糖治疗组大鼠脑内的多巴胺、去甲肾上腺素、5-羟色胺、酪氨酸羟化酶与抑郁组、生理盐水组大鼠脑内的含量均明显升高,差异有统计学意义(P0.05)。结论:灵孢多糖注射液对改善抑郁症状有良好的疗效,具有辅助治疗抑郁症的作用。

The Assisting Effect and Mechanismof Ganoderma Lucidum Polysaccharides in Treatment of Depression

Objective:To establish the depression model of rats, and investigate the function and mechanism of Ganoderma lucidum Polysaccharides on the treatment of depression.Methods:Animal experiments: Forty-eight SD rats were randomly divided into six groups: normal control group, depression group, saline solution group, ganoderma lucidum polysaccharides treating group(low-dose, moderate-dose, high-dose) and chronic stress -induced depression. Behavioral indicators were used to evaluate the modeling success. Then we injected with low , medium and high doses of the Spirit spore polysaccharide in Spirit spore polysaccharides groups, and the different effects with the different groups were compared. Meanwhile, immunohistochemical method was applied to detect the expression of monoamine neurotransmitters, such as dopamine, Norepinephrine, Serotonin and tyrosine hydroxylase, in the rats'' brain.Results:The models of depression in rats were successfully established. The behavior indicators of depression group were lower than those in the control group (P<0.05). And the indicators significantly increased after drug treatment. The expressions of monoaminal neurotransmitters of ganoderma lucidum polysaccharides group were much higher than those in the depression group and saline solution group (P<0.05).Conclusion:Ganoderma lucidum polysaccharides has better effect on improving the symptoms of depression, it have some assisting effect on depressive treatment.