NAIF1对肝癌细胞 HepG2 增殖与迁移能力的影响

目的:在肝癌细胞Hep G2中过表达外源NAIF1(核凋亡诱导因子1),探讨NAIF1的亚细胞定位以及对Hep G2增殖和迁移能力的影响。方法:以真核表达质粒p EGFP-N1为对照组,p EGFP-N1-NAIF1为实验组,瞬时转染肝癌细胞Hep G2,利用免疫印迹方法检测NAIF1蛋白表达效率;以DAPI染核,荧光显微镜下观察绿色荧光蛋白定位,确定NAIF1的亚细胞定位;通过MTT方法绘制细胞增殖曲线;通过transwell小室法检测NAIF1对Hep G2迁移能力的影响。结果:在肝癌细胞Hep G2中,外源表达NAIF1主要定位于细胞核;与对照组Hep G2/p EGFP-N1相比,Hep G2/p EGFP-N1-NAIF1的细胞增殖、迁移能力下降(P<0.05)。结论:外源表达NAIF1蛋白定位于Hep G2细胞核,过表达NAIF1抑制Hep G2的细胞增殖与迁移能力,NAIF1可能作为肝癌治疗的潜在靶点。

Effects of NAIF1 on Cell Proliferation and Migration in Hepatoma Cell HepG2

Objective:Exogeneous (nuclear apoptosis-inducing factor 1 ) was transfected and expressed in hepatoma cell HepG2, and the localization of as well as the effects of on cell proliferation and cell migration was investigated.Methods:HepG2 cell was transiently transfected with eukaryotic expression plasmid pEGFP-N1 as control group or pEGFP-N1- as test group. Western blot was performed to examine the protein expression levels. Nucleus was stained by DAPI, and the localization of was analyzed by monitoring the GFP using a fluorescence microscope. The ability of cell proliferation was determined by MTT assay. Transwell assay was performed to examine the ability of cell migration.Results:In hepatocma cell HepG2, exogeneous expressed was mainly localized in nucleus. Compared with HepG2/pEGFP-N1 , HepG2/pEGFP-N1- showed decreased ability of cell proliferation and migration (P<0.05).Conclusion:Exogeneous expressed localizes in the nucleus of HepG2. Overexpressed inhibits the cell proliferation and migration of HepG2. may act as a potential target of hepatocarcinoma therapy.