一种白细胞介素 -12 缓释微球制备及体外表征

目的:开发一种白细胞介素-12(IL-12)长效缓释微球剂型。方法:采用水包油-油包固(S/O/W)法制备了白介素-12因子多糖微粒的聚乳酸-聚羟基乙酸共聚物(PLGA-PLA)微球,研究了微球的表面形态和粒径大小,并且运用ELISA方法考察了微球的体外释放效果和免疫活性。结果:本方法制备的白介素-12因子微球光滑圆整,体外缓释达45天,累积释放率近90%。结论:本方法制备的白介素-12因子微球,不仅具有有效地保护IL-12蛋白活性,同时实现长效缓释的目标,是一种可行的IL-12缓释方案。

Study on Preparation and Vitro Characterization of Interleukin-12 Sustained-Release Microsphere *

Objective:To exploit an interleukin-12 (IL1 -2) long-term sustained release microsphere formulations.Methods:Dextran glassy particles were prepared by a unique method of low temperature aqueous-aqueous "emulsion", and then the IL-12-loaded dextran glassy particles were encapsulated into PLGA microspheres by the method of solid-in-oil-in- water (S/O/W) emulsion-evaporation technique. The microspheres were characterized by SEM. In vitro release profiles of IL-1 2 loaded microspheres were plotted by Human IL1 2 Quantikine ELISA Kit.Results:The IL-1 2-loaded microspheres possessed spherical shape and smooth surface. The cumulative release in vitro was about 90% after 45 days.Conclusion:This method effectively protected the bioactivity of IL-1 2 and microspheres exhibited an ideal sustained release behavior.