| Ⅰ型单纯疱疹病毒感染的细胞培养系统的建立 |
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| 引用本文： | 梁义,林有坤,梁铭.Ⅰ型单纯疱疹病毒感染的细胞培养系统的建立[J].现代生物医学进展,2010,10(5). |
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| 作者姓名： | 梁义 林有坤 梁铭 |
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| 作者单位： | 1. 柳州市柳铁中心医院皮肤科,广西,柳州,545007
2. 广西医科大学第一附属医院皮肤科,广西,南宁,530021 |
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| 基金项目： | 广西自然科学基金资助(桂科0135036) |
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| 摘 要： | 目的:建立稳定的HSV-1感染的细胞培养系统,为HSV-1感染性皮肤病的基础及临床研究提供稳定的平台。方法:以猴肾细胞(Vero)为繁殖细胞,选择人鼻咽癌上皮细胞(Hep-2)为靶细胞,观察HSV-1在Hep-2株中的致细胞病变效应(CPE)。结果:HSV-1在Vero细胞中能稳定、大量地繁殖;HSV-1感染Hep-2细胞后可出现明显的细胞病变;结论:以Vero为繁殖细胞,Hep-2为靶细胞的稳定的细胞培养系统可作为HSV-1感染性皮肤病的基础及临床研究的平台。
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| 关 键 词： | 单纯疱疹病毒1型 细胞培养 猴肾细胞 人鼻咽癌上皮细胞 |
Establishment of HSV-1 Infected Cell Culture System |
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| LIANG Yi,LIN You-kun,LIANG Ming.Establishment of HSV-1 Infected Cell Culture System[J].Progress in Modern Biomedicine,2010,10(5). |
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| Authors: | LIANG Yi LIN You-kun LIANG Ming |
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| Institution: | LIANG Yi1,LIN You-kun2,LIANG Ming2.(1 Department of Dermatology,Liu Zhou Railway Central Hospital,Liu Zhou,Guangxi 540047,China,2 Lin Youkun,Liang Ming.Department of Dermatology,First Affiliated Hospital,Guangxi Medical University,Nanning,Guangxi530021,China) |
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| Abstract: | Objective:To establish a stable HSV-1 infected cell culture system,in order to provide a stable platform for further basic and clinical research of skin disease caused by HSV-1 .Methods:HSV-1 strains were propagated in vero cells and hep-2 cells were served as targets of HSV-1 infection.Then cytopathic effect (CPE) was performed and observed.Results:Abundance of HSV-1 were stable propagated in Vero cells;The CPE occurred obviously in hep-2 cells after infected by HSV-1.Conclusion:The HSV-1 infected cell cul... |
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| Keywords: | HSV-1 Cell Culture Vero Hep-2 |
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