| PCR 防污染技术的研究进展 |
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| 引用本文： | 王璐茜,邹秉杰,周国华.PCR 防污染技术的研究进展[J].现代生物医学进展,2011,11(14):2797-2800. |
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| 作者姓名： | 王璐茜 邹秉杰 周国华 |
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| 作者单位： | 1. 中国药科大学生命科学与技术学院,江苏南京,210009
2. 中国药科大学生命科学与技术学院,江苏南京210009;华东医学生物技术研究所,江苏南京210002 |
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| 基金项目： | 江苏省自然科学基金(No.BK2010111); 国家自然科学基金项目(No.21005088) |
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| 摘 要： | 聚合酶链式反应(PCR)是一种高灵敏核酸扩增技术,广泛应用于核酸检测中.但在实际应用过程中,扩增产物及其他核酸片段的污染会导致假阳性的结果,制约了PCR在临床检测中的应用.为了解决这一问题,建立了许多PCR防污染的方法,除了早期建立的并已得到广泛应用的物理隔绝法、光照法及水解法外,近年来还发展了酶消化法、化学修饰法及DEAE纤维素法.本文对PCR防污染技术的原理、应用及进展进行了综述.
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| 关 键 词： | PCR 防污染 UNG DNaseI 限制性内切酶 |
Research Progress of PCR Decontamination Methods |
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| WANG Lu-qian,ZOU Bing-jie,ZHOU Guo-hu.Research Progress of PCR Decontamination Methods[J].Progress in Modern Biomedicine,2011,11(14):2797-2800. |
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| Authors: | WANG Lu-qian ZOU Bing-jie ZHOU Guo-hu |
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| Institution: | WANG Lu-qian1,ZOU Bing-jie1,ZHOU Guo-hua1,2(1 College of Life Science and Technology,China Pharmaceutical University,Nanning 210009,China,2 Huadong Research Institute for Medicine and Biotechnics,Nanning 210002,China) |
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| Abstract: | Polymerase chain reaction(PCR) is a highly sensitive nucleic acid amplification technique,which has been widely used in nucleic acid detection.However,the contamination of amplicons or other nucleic acid fragments,which usually leads to false-positive results,limits it's clinical applications.To solve this problem,many approaches were developed to eliminate PCR contami-nation.Besides early established methods based on physical separation,irradiation and hydrolysis,methods by using enzyme digestion,chemical ... |
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| Keywords: | PCR Decontamination UNG DNase I Restriction endonuclease |
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