基因沉默孤儿核受体ERR-alpha抑制前列腺癌细胞的体内转移 |
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引用本文: | 肖利佳,郝建华,李江,李钢,王娟,邹畅.基因沉默孤儿核受体ERR-alpha抑制前列腺癌细胞的体内转移[J].现代生物医学进展,2015,15(10):1855-1857. |
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作者姓名: | 肖利佳 郝建华 李江 李钢 王娟 邹畅 |
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作者单位: | 广东医学院附属南山医院检验科;暨南大学第二临床医学院,深圳市人民医院临床研究中心 |
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基金项目: | 广东省深圳市南山科技局项目(2012005) |
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摘 要: | 目的:研究孤儿核受体ERRα对前列腺癌细胞E-cadherin(上皮细胞钙粘蛋白)的表达水平和体内转移能力的影响。方法:利用慢病毒介导的sh RNA构建稳定下调ERRα表达的DU145-sh ERRα和PC-3M-sh ERRα前列腺癌细胞模型,同时用ERRα特异性抑制剂XCT790抑制其活性,并利用Western Blotting(免疫印迹)检测上皮细胞标志物E-cadherin的表达水平。将PC-3M-sh ERRα细胞和PC-3M-scramble对照细胞用荧光素酶标记后原位注射小鼠前列腺,8周以后通过体内成像系统检测原位瘤的形成及其体内转移情况。结果:基因沉默ERRα表达水平和用其特异性抑制剂XCT790处理DU145后,E-cadherin的表达水平明显降低。在PC-3M-sh ERRα细胞中,E-cadherin的表达水平明显低于对照组,同时由其构建的6只原位前列腺癌小鼠模型中没有发生转移,而由对照组细胞构建的7只原位前列腺癌小鼠模型中有4只发生了转移。结论:在前列腺癌细胞中下调ERRα的表达水平抑制其E-cadherin的表达和体内转移能力。
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关 键 词: | ERRα E-cadherin 前列腺癌 体内转移 |
Knockdown ERR-alpha Inhibits in Vivo Metastasis in Prostate Cancer Cells |
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Abstract: | Objective:To explore the roles of orphan nuclear receptor ERR-alpha in E-cadherin expression regulation and in vivo
metastasis in prostate cancer cells.Methods:DU145-shERR-alpha and PC-3M-shERR-alpha cell models were established by gene knockdown
mediated by lentivirus. ERR-alpha specific antagnist XCT790 was used to inhibit its activity, the expression level of`E-cadherin was
identified by Western Blotting. PC-3M-shERR-alpha and PC-3M-scramble cells were labeled by luciferase and injected in situ in mice
prostate, metastases were measured through in vivo image system and indicated by the fluorescence intensity 8 weeks after injection.Results:The expression level of E-cadherin was significantly decreased in DU145 after knockdown ERR-alpha or treatment with XCT790.
The expression level of E-cadherin in PC-3M-shERR-alpha was significantly suppressed as compared to that in control cells. Moreover, 4 of
7 in situ prostate cancer mice models dreived from PC-3M-scramble cells, while 0 of 6 from PC-3M-shERR-alpha cells, develop metastases.Conclusion:Knockdown ERR-alpha in prostate cancer cells attenuate E-cadherin expression and their in vivo metastasis. |
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Keywords: | ERR-alpha E-cadherin Prostate cancer In vivo metastasis |
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