| Fmoc固相合成JFT的工艺研究 |
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| 引用本文： | 唐宏琨,孙立枢,陈五岭.Fmoc固相合成JFT的工艺研究[J].现代生物医学进展,2007,7(1):85-87. |
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| 作者姓名： | 唐宏琨 孙立枢 陈五岭 |
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| 作者单位： | 西北大学生命科学院,陕西,西安,710069 |
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| 摘 要： | 目的:研究多肽JFT的合成工艺。方法:本实验采用固相合成法(spps),以Fmoc—氨基酸为原料,TBTU\HoBt\DIEA混合试剂缩合,用三氟乙酸\苯甲硫醚\巯基乙醇\苯酚\水脱保护,将多肽从MBHA树脂上切割下来。结果:粗肽的收率为62%,经RP-HPLC纯化,即可获得纯度在98%以上的目标肽。经MALDI—MS质谱分析其分子量与理论值一致。结论:此工艺操作简单,便于推广,适合大规模生产。
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| 关 键 词： | 固相合成多肽 Fmoc-氨基酸 Rink-Amide-MBHA树脂 分离纯化 |
| 文章编号： | 24081141 |
| 修稿时间： | 09 14 2006 12:00AM |
Study on Solid-phase Synthesis of JFT Peptide |
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| TANG Hong-kun,SUN Li-shu,CHEN Wu-ling.Study on Solid-phase Synthesis of JFT Peptide[J].Progress in Modern Biomedicine,2007,7(1):85-87. |
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| Authors: | TANG Hong-kun SUN Li-shu CHEN Wu-ling |
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| Abstract: | Objective:Studing on the technology of synthesis of JFT.Methods:It was synthesized with solid-phase peptide synthesis on Fmoc Rink-Amide-MBHA resin,using Fmoc-aa\TBTU\HoBt\DIEA activation system.After cleaved with TFA\Thioanisole\Mercaptoethanol\Phenol\H_2O the peptide yidld was 62%.Results:After purification with semipreparative RP- HPLC,the purity was found more than 98%.It was identified on MALDI-MS and have the same number with the theoretical one. Conclusion:This method is simple and convenient to be expended and popularized. |
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| Keywords: | Solid-phase peptide synthesis Fmoc-aa Rink-Amide MBHA resin Separation and purification |
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