不同分化状态下间充质干细胞向血管内皮生长因子的迁移

目的:探讨间充质干细胞(MSCs)对趋化因子VEGF的定向迁移能力与其分化状态之间的关系。方法:本实验运用采用Percoll分离法在体外培养并扩增大鼠骨髓来源MSCs,应用抗氧化剂诱导方案诱导MSCs向神经样细胞分化,运用Boyden chamber及Dunn chamber趋化性迁移装置研究了在趋化因子VEGF诱导下不同分化状态的间充质干细胞定向迁移,比较了各分化状态下细胞的迁移速度和迁移效率。结果:Boyden chamber实验结果显示下室加入不同浓度VEGF后,不同状态细胞向同一浓度VEGF迁移的数量不同,不同浓度VEGF诱导同一状态细胞的迁移数量也不同;Dunn chamber的实验结果显示在某一分化阶段(预诱导24小时)的MSCs具有更高的迁移效率。结论:MSCs的分化影响了其向VEGF的定向迁移,也就是说,不同分化状态的MSCs显示出不同的迁移行为。

The Migration of Differenting MSCs towards VEGF

Objective:To investigate the relationship between VEGF-induced chemotactic migration of MSCs and their differentiation states.Methods:MSCs were isolated by Percoll gradient centrifugation from rat and induced to neuron-like cells. Then, Boyden chamber and Dunn chamber were used to study the behavior of direct migration fromthe population level and single cell level respectively.Results:Data from Boyden chamber experiment revealed that the migration number of cells differed among the differentiation states and the concentration of VEGF also affected the number of migrating cells. Meanwhile, time-lapse video analysis revealed MSCs at certain stage (24 h pre-induction) possess higher FMI (forward migration index).Conclusion:Differentiation of MSCs influences their chemotactic responses to VEGF.